Methylglyoxal: Large Response Rate in Very Advanced Stages

Introduction:

Methylglyoxal, a molecule also produce by human body, it is one of the substances with an anti cancer potential that stands out.

I am enthusiast about it because of the following facts:

  1. It has clear science behind supported by a large amount of academic research
  2. Its anti cancer potential has been already demonstrated in humans
  3. It can eradicate most cancer types
  4. Its anti cancer action was even indicated by a Nobel Prize winner in multiple publications in the prestigious magazine “Science”
  5. It is cheap
  6. Relatively easy accessible and can be administrated orally
  7. Low to no toxicity to normal cells

I will start this article with a number of major historical facts indicating the potential behind Methylglyoxal:

  • At the begging of the last century …
    Dr. William F. Koch (1885-1967) (appointed Professor of Physiology at the Detroit College of Medicine in 1914, and subsequently became Chairman of that Department) published a paper entitled “A NEW AND SUCCESSFUL TREATMENT AND DIAGNOSIS OF CANCER”. In this paper he was stating the following: “This substance when purified, taken up in water and immediately injected subcutaneously into a cancer patient, causes practically no local reaction; but instead, after about 24 hours, a very decided focal reaction takes place. Wherever the cancer tissue may be, its’ cells are killed, their ionic concentration increases, the osmotic pressure increases, they take up water, swell and disintegrate. The swelling causes pain and the absorbed, disintegrated products are oxidized causing fever.”“Those two things, focal pain and fever, constitute a reaction which lasts all the way from 6 to 48 hours, depending upon the amount of cancer tissue killed, and of course this depends upon the quantity of substance injected. Such a reaction occurs only in cancer cases and only in the presence of cancer tissue. After the cancer tissue has disappeared, no more reaction can be elicited, no matter how large an injection is given, an important diagnostic aid. The specificity of the substance for cancer is evidenced by the fact that while giving these injections in rapid succession (that is daily or every two days for a period of five weeks), a blood count will rise from 2,850,000 to 4,600,000 red cells and the haemeglobin from 37% to 82%. Thus the delicate red cells are not injured. At the same time a mass of cancer tissue, the size of a large cabbage, will entirely disappear, and all the signs and symptoms of the particular cancer will disappear with it, function return, and the patient become clinically cured.“Stomach, liver and rectal cancers clear up the quickest. Uterus cancer responds slightly more slowly. Squamous cell carcinoma responds about one-half as fast as stomach cancer.” (Ref.)With his therapy W Koch successfully treated various cancer types. Here are a few examples of successful cases http://www.williamfkoch.com/web/version2/drkoch.php?id=151.0&dispID=disp(2);%20disp(3). There is so much to say about what happen following the success of Prof. Dr. William F. Koch, and at this link you can read all in details, but on short, unfortunately for the general health, Dr. Koch was never given the research facilities and cooperation by the medical profession he had asked for and wanted.
    .
    Here are a few pieces of information that can give us a view on the drug used by Dr. Koch:
    – Once, Dr. Koch stated about his anti cancer substance the following: “The compound is difficult to make and it deteriorates rapidly. If I published it and quacks or unscientific men started mixing it and treating cancer with it, the results would be disastrous, not only possibly to the patients, but to the ultimate success of the treatment. Improperly mixed or administered the compound would fail to do its work.” (Ref.)
    – In another article this is what I found: It was an oxygen uptake product of some kind. The formula was O = C = C = O. According to the formula that Koch published it was a 10e-6 dilution, i.e. homeopathic formulation. He called this substance Glyoxylide (Ref.)
    – And here is another piece of info on his formula: The theory that Koch had was one in which he equated the dilution of his product to enzymes in the blood
    .
    So, despite offers, he never made the formula for his medicine available to medical groups or drug companies, for fear they would change and pervert it.
    .
    The substance that Koch suggested he was using was O = C = C = O, and it was considered essentially nothing by FDA. Her is a quote “The formula was O = C = C = O, which means nothing, really” (Ref.) As a result, his drug was classified as a fraud by the FDA (Ref.).
    .
    quackwatch.com is stating the following about Koch’s glyoxylide: “Does Koch’s glyoxylide exist? The molecule glyoxylide has been a subject of investigation by chemists including H. Staudinger in 1913 [4] to Berson in 1986 [5]. Recently Sulzle [6] reviewed the literature and considered the theoretical possibilities for the existence of a compound like glyoxylide. He found that all efforts to prepare, isolate, or chemically identify this compound failed. His studies on the theoretical physical chemistry of glyoxylide showed that the substance described by Koch cannot exist in nature. This, along with Jenssen’s failure to find anything in Koch’s “medicine” [3], confirms the conclusion that the glyoxylide which Koch claims to have invented did not exist.” However
    .
    … Nearly 100 years latter, during 2015, here is a headline from the scientific news: “Existence of elusive molecule confirmed after more than a century”. (Ref.) This shows how scientist at University of Arizona, USA, have discovered a small molecule with only four atoms that was “never been observed, neither as a substance nor as a transient species, despite a century-long history of attempts”. And guess what was the molecule discovered? That was exactly O = C = C = O (OCCO), the same that dr. Koch was suggesting he was using to treat cancer and FDA was saying that it doesn’t exist :). This simple molecule was so hard to find because it splits into two carbon monoxide (CO) fragments after half a nanosecond or so of existence. The discovery was made in Sanov’s lab, when his graduate students were experimenting with glyoxal – a chemical compound with the formula OCHCHO. (Ref.). So Koch’s OCCO precursor is actually glyoxal. OCCO is now called ethylenedione. The relation between Koch’s work and Glyoxal is also shortly discussed here.
    .
    This proves the fact that if you don’t see it doesn’t mean automatically that it doesn’t exist. It also means, quackwatch and FDA were wrong and need to update their statements on the above.
    .
    Therefore, Koch succeeded to find a way to synthesizing OCCO and keep it stable somehow until giving it to the patient or use substances that would trigger its production in the human body (Ref.), and with this cure cancer and other diseases. For more information on Koch’s story please see williamfkoch.com, a site maintained by the Koch family.
    .
  • In the middle of the last century …
    Dr. Albert Szent-Gyorgyi
    was the Nobel Laureate in Medicine in 1937 for the isolation and discovery of Vitamin C. (Ref.) he also discovered Iso-Flavones and vitamin P. In his last 40 years, he researched the regulatory processes of cell growth, and thereby the regulation of cancer itself.
    .
    As early as 1958, Szent-Györgyi also worked on Methylglyoxal and together with his collaborators, in their pioneering work on the biological role of Methylglyoxal, had put forward strong evidences for the anti-cancer and tumor growth inhibitory effect of Methylglyoxal. For example, they showed that Methylglyoxal could completely inhibit the tumor development in mice. Here is the ‘Science’ paper published in 1968: Cancerostatic Action of Methylglyoxal.
    .
    Even earlier than that, in 1963, the prestigious magazine ‘Science’ published a remarkable article about his research. In it Dr. Szent-Gyorgyi identified two substances, one called Retine, which inhibited cancer growth (ascites tumors), and the other called Promine, which promoted cell growth and made cancer grow faster. (Ref.) He suggested that these were very small molecules that were highly potent in controlling cell division. His research using mice achieved shrinkage of tumors by increasing the ratio of Retine to Promine with daily injections of Retine. Other researchers obtained similar results and there were no harmful or toxic side effects.
    .
    In another article in Science he announced that they have succeeded to extract Retine from human urine. (Ref.) I find this interesting since urine therapy as an anticancer approach was/is a technique used by some. And here is another article referring to glyoxal offering a hopeful target in the search for cancerostatic substances (Ref.) and more on his view on methylglyoxal (Ref.) In 1967 he announced that his laboratory had isolated and manufactured Retine (retards cell growth) in the form of a Carbonyl compound called Methylglyoxal. (Ref.)
    .
    In an interview in Prevention magazine in 1972 conducted by Jane Kinderlehrer, he explained that he and “Dr. Egyud have found that retine (methylglyoxal) stops the growth of cancer cells without poisoning other cells. When retine is present in sufficient concentration, no cell division can occur while vital cellular processes go on unhindered. And what is a good bit of luck, and not my cleverness, the white-haired scientist pointed out, is that if a cancer cell cannot grow, it dies by itself.” According to the researchers, retine is normally produced by the body and, when it is, it prevents the growth of existing cancer cells. But the body can lose its ability to produce this substance… “Putting the retine back in the body, just as we put insulin back into a diabetics body, can stop the growth of cancer… ” (Ref.)
    .
    Dr. Szent-Gyorgyi acknowledged the work of Dr. William Koch on the same subject saying, “A decade ago, a very intuitive researcher, Dr. William F. Koch, came to the same conclusion about the possible importance of Carbonyls in regulation of cell division and carcinostasis.”
    .
  • At the end of the last century and beginning of our century …
    About 50 years latter Prof. dr. Manju Ray, a very good Indian scientist in Molecular Enzymology and Cancer Biochemistry, build on Methyloglyoxal’s anti cancer effects. In a series of papers, she further demonstrated the potential of Methyloglyoxal. But she did not stop to the theory. Instead she trialed the drug on 19 patients with very advanced stages of cancer and resulted in an overall cure rate of 70 percent in cancer patients who were diagnosed as terminally ill, results presented in 2001.
    .
    In 2006 Prof. Dr. Manju Ray, presented a 5 year follow-up phase II study with methylglyoxal. It showed that of the 46 patients enrolled, 18 were in complete remission (so there were no tumors in clinical scans to see). The follow-up after 5 years of the patients took an average of 4 to 56 months. The results of the study showed that 18 (39%) patients achieved a complete remission, 18 (39%) patients had partial regression and / or stable disease, while 8 (17%) patients had progression of their disease.” After this, another successful clinical trial was conducted (see below).
    .
    With all these results in humans, Prof. Ray clearly underpinned the great anticancer effect of Methylglyoxal.

Other facts:

  • Methylglyoxal can also enable or increase the effectiveness of DNA disrupting chemo therapy (Ref.).
  • Ketogenic Diet seems to also increase intracellular Methylglyoxal (Ref.)
  • Methylglyoxal can be also found in Manuka Honey in concentration of about 250mg/kg and it is jar of honey with honeycomb on wooden tablebelieved to be responsible for the antibacterial and antiviral activity of the honey. (Ref.) Professor Thomas Henle of University of Dresden, Germany announced in 2008 that research “unambiguously demonstrates for the first time that Methylglyoxal is directly responsible for the antibacterial activity of Manuka honey.” http://www.manukaonline.com/mgo-Manuka-Honey-benefits.html
  • However, note that while the amount of Methylglyoxal  present in the honey (250mg/kg) may serve as a good preventive measure,  for cancer treatment purpose this is too little. Given the Methylglyoxal  dose that is proposed to cure cancer (see the Dose and Administration section below), we would need to eat kilograms of honey each day, which is not feasible. This is why, as it will be further discussed, other sources of Methylglyoxal  are used in the clinical trails.

Other interesting articles to read:

In conclusion, the anticancer effect of methylglyoxal has been known for a long time. But relatively recent work has shown that it acts exclusively against malignant cellular mitochondrial complex I and GAPDH to elicit its anticancer effect. It has been used on humans and found that

  • methylglyoxal is potentially safe for human consumption and able to destroy cancer cells in vivo
  • a methylglyoxal-based anticancer formulation was administered orally to diverse groups of cancer patients in India
  • In first group (14 months, January 2000-February 2001) 24 patients were recruited and complete remission was observed for 11 patients and partial remission for 5 patients.
  • In the second group (60 months, October 2000-September 2005) 46 patients were recruited and complete remission was observed for 18 patients and partial remission for 18 patients.
  • In the third group (42 months, May 2005-October 2008) of the 23 patients complete remission was observed for 11 patients and partial remission for 7 patients.
  • The treatment was found to be especially effective for adenocarcinoma of urinary bladder, breast, uterus, esophageal and gastrointestinal tract cancer.
  • Several vital biochemical, radiological and other parameters were tested in patients who received treatment for a long time to assess the possible long term toxicity of methylglyoxal treatment, if any, and the results implicated no toxicity as per the parameter studied.
  • All the results showed great promise of methylglyoxal treatment and demands further improvisation the methylglyoxal based therapeutics. (Ref.)
  • Due to its mechanims of action it could complement anticancer strategies involving Chemo, 3BP, DCA, Artemisinin, and any other pro oxidant drug.

Methylglyoxal as anti cancer treatment in Humans: Clinical trials

A methylglyoxal-based anticancer formulation was developed and a three-phase study of treating a total number of 86 cancer patients was carried out. The results appear to be promising. Most of the cancer patients benefited greatly and a significant number of patients became free of the disease. Contrary to the effect of existing anticancer drugs, this methylglyoxal-based formulation is devoid of any toxic effect and reasonably effective against a wide variety of cancers. (Ref.)

Here are the result from one of the study 46 patients:

Previous in vitro and in vivo studies had shown remarkable anticancer effect of methylglyoxal. A recent toxicological study with four different species of animals has shown that methylglyoxal is potentially safe for human consumption (Ghosh et al, 2006). We have developed an anticancer formulation with methylglyoxal as the principal ingredient. To test the efficacy of this formulation, 46 patients suffering from different types of malignancies in different stages of the disease were randomly chosen: brain –2, head and neck –2, gastrointestinal –11, lung –6, gynecological –6, breast –3, urological –4, hematological –2, prostate –2, gall bladder –1, pancreas –2, others –5. The effect of the formulation on overall survival, regression of the tumours and general well being of the patients were analyzed. The follow-up of the patients ranged from 4–56 months. The results of the study show that 18 (39%) patients had complete remission, 18 (39%) patients had partial regression and/or stable disease condition, whereas 8 (17%) patients had progressive disease. In addition to the measurable improvement of the majority of the patients there was remarkable improvement in the quality of life of nearly all the patients. There was no significant adverse side effect in almost all the patients. The significant antitumour effect of methylglyoxal against a wide variety of cancer suggests that all the different types of cancer may have common altered site(s). Our next task will be to further improve this treatment and to evaluate its efficacy with a large number of patients. http://www.cancer-therapy.org/CT/v4/B/HTML/17.%20Talukdar%20et%20al,%20205-222.html

Clearly, the results are great as they indicate 78% response rate, including 39% complete remission.

Anecdotal stories

  • a patient in the Netherlands keeping his cancer under control from 2001 to 2005 with MG. Doctors recognizing the patient would not be there in 2005 without MG (Ref.)

Mechanism:

Methylglyoxal (MG) is a highly reactive dicarbonyl compound and a potent glycating agent, mainly generated as a by-product of glycolysis through a spontaneous degradation of triosephosphates. In cancer, since the glycolytic pathways is highly active there is a lot of Methylglyoxal being produced. That is even more during the administration of DNA disrupting agents such as some of chemotheraphies (see next paragraph). Figure below shows how Methylglyoxal is produced inside the cell.

Figure is from: Analysis of methylglyoxal metabolism in CHO cells grown in culture

MethylglyoxalThe cellular response to antitumour drugs that modified DNA or disrupt DNA metabolism is to activate processes of DNA repair, including poly(ADP-ribose) polymerase. This depletes cells of NAD+ such that glyceraldehyde-3-phosphate dehydrogenase activity is depleted and triosephosphates, glyceraldehyde-3-phosphate and dihydroxyacetonephosphate, increase. Methylglyoxal is formed mainly by triosephosphate degradation and since triosephosphates is increased strongly during the DNA repair, a consequent dramatic increase in methylglyoxal formation is expected. The increase of methylglyoxal will be negative for cancer cell potentiating for example the cytotoxic effect of the antitumour agents. As a result, to block methylglyoxal, the cancer cell will over express of Glo1 (Ref.)

Indeed, it has been shown that in mammalian cells, MG is detoxified by the glyoxalase system, an enzymatic pathway consisting of two enzymes called glyoxalase 1 (Glo-1) and glyoxalase 2 (Glo-2) and is based on reduced glutathione (GSH). It has been shown that Glo-1 expression and activity is increased in many human cancer types such as colon, prostate, melanoma, lung, and breast and that Glo-1 overexpression is correlated with cancer progression and drug resistance (Ref.).

Accumulation of Methylglyoxal in cancer cells are known to lead to the inhibition of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Ref1Ref2, Ref3), an essential enzyme acting in the glycolsisis pathway. GAPDH inhibition depletes ATP profoundly depriving the cancer cells of energy. Note that 3-bromopyruvate (3BP) is also inhibiting (GAPDH) (Ref1., Ref2.).

Next to GAPDH inhibition, Methylglyoxal induces mitochondria-dependent apoptosis http://www.ncbi.nlm.nih.gov/pubmed/22098242

One of the potential anticancer mechanisms of MG may be related to GABA A receptor. This is because MG seems to be an activator of GABA A receptor (Ref.). And GABA is known to show anti cancer activity via the GABA A receptor (Ref.1, Ref.2).

As a side note the activation of GABA A receptor triggers an influx of Cl- ions (Ref.).

Dose and Administration:

MG oral administration:

According to the clinical trial above (Ref.):

  • 30mg methylglyoxal/kg of body weight/day
  • this was divided in 4 administration/day  (i.e. 7.5mg/kg/administration)
  • each dose was followed by a tablet of chewable vitamin C containing 400 mg of sodium ascorbate
  • Taking the drug on an empty stomach is NOT recommended
  • Each patient also received orally a mixture of the B vitamins twice a day: B15mg, B6 2.5 mg, B12 5 mg and B5 7.5 mg. This mixture is usually a standard composition of vitamin B complex available
  • The duration of treatment at the same or at a reduced dose was determined by evaluating the response and general condition of the patient

Now here is how to translate the above dose into what the chemical suppliers are selling:

  • Sigma and others provide 40% MG concentration in water
  • The density of MG is 1.17g/ml at 25C; this means that each ml of 40% MG contains 468mg MG
  • Since the dose suggested for a day is 30mg/kg, a person of 50kg will need 1500mg MG/day
  • Since each ml of 40% MG contains 468mg MG, the 50kg patient would need 3.2ml of 40% MG each day
  • As a result 0.8ml of 40%MG has to be administrated 4x/day
  • As suggested in the clinical trial, each of the 0.8ml will be administrated with 60ml water (i.e. in a small glass of water) after meal

Note: in addition to Vitamin B and Vitamin C suggested in the clinical trial I would also add Curcumin capsules (8g/day or lower if this is not possible) to inhibit glyoxalase 1 and possibly increase the effectiveness of MG treatment. Here is a patent that is suggesting the same https://www.google.com/patents/US8163796

15.05.2016: Update on formulation and dose: I was just informed by one of the readers in contact with dr. Ray that in case of very aggressive cancers she is now suggesting 40mg/kg/day dose. Here is a quote of the info I received: “40 mg/Kg/day. 5 ml of her formulations has to be taken with half a cup of water/fruit juice after meal/snack four times a day, followed by one 500 mg chew-able Vitamin C (2000 mg daily total), and two tablet Polybion after lunch and dinner.

23.05.2016: Update on formulation, dose and administration following conversation with Prof. Manju Ray:

  • Methylglyoxal (MG) used is available commercially as 40% aqueous solution
  • We now use 40 mg/kg/day in four divided doses
  • For example a person of 50 kg will need 2000 mg MG in four divided
    doses/day
  • 40% methylglyoxal means 40 gm/100 ml (irrespective of density)
  • Take 100 ml of 40% solution, add 300 ml of water (preferably distilled water)
  • Total vol of diluted solution is 400 ml
  • 400 ml contains 40000 mg,
  • so a person of 50kg that needs 2000 mg/day will use 20ml of this diluted solution every day, in 4 devided doses, each of 5ml
  • Mix 5 ml of diluted solution with 50-75 ml of water or fruit juice and give the patient to drink
  • Such a dose should be given 4 times daily in 5-6 hrs intervals
  • After each dose of methylglyoxal,  1 tab of chewable Vit C = 500 mg should  be chewed (not swallow)
  • tablet of Polybion after lunch and dinner is OK
  • Curcumin is OK
  • My addition: Creatine supplement is a must 30-60 min prior to MG administration (see below why)
When reading the patent on Methylglyoxal I found the following statement:
“Moreover, it was observed that creatine present in cardiac cells completely protected the animal from any possible deleterious effect of methylglyoxal treatment on cardiac mitochondria (Sinha Roy et al., 2003).” http://www.google.com/patents/US20110118327 Also another similar statement is here: “Furthermore, creatine can protect cardiac mitochondria from the deleterious effects of some anticancer compounds” http://publicationslist.org/data/theo.wallimann/ref-253/Ray-CK_Creatine_anti-cancer.AAS-2011.pdf
Although in the clinical studies they did not used Creatine, following these statements, I would clearly make sure that (maybe 30-60 minutes) prior to each MG administration there is a Creatine supplement administer too.
Next to supporting MG treatment Creatine alone seems to have both anti cancer and anti cachexia effects (Ref.)
.
Update on 21-June-2016:

MG IV administration:

Based on recent discussions with prof Manju Ray here is a dose that makes sense:

– 10mg/Kg MG body weight/day (sterilized with a 0.2um sterile filter as discussed in other posts on this website)
– divide this in two administrations and administer each in 12 hours interval (e.g. one to start at 9:00 a.m. and the other to start at 9:00 p.m.)
– each administration contains 5mg/kg in 250ml NaCl and is given in 3 hours
.
Update 22-June-2016:

Koch formulations administration:

“A successful product if taken sublingually will be tasteless at first. Then within a few minutes a slightly astringent (cotton mouth) feeling will be experienced followed by a metallic taste. Many will go on to experience a transient warm flushed feeling several minutes later, and/or feelings of increased pep and mental alertness. Those subjects having allergic symptoms will usually experience relief within a few minutes. If one or more of the above occur, the product should be considered good. The product need not be injected, but can be administered sublingually or by aerosol.”http://bioredox.mysite.com/OCThtml/howmade.htm

Koch formulations are:

It is recommended to be given

  • 1 Vial per day for 10 days- then –
  • 1 Vial per week for 10 weeks- then –
  • 1 Vial per month for the balance or until vials are gone. (Ref.)

Other sources are suggesting

  • 1 Vial each day during 14 days

Regardless of the approach, the Vials are alternated, e.g. first day one vial Rhodizonsaure, second day one vial Carbonylgruppen, next day again one vial Rhodizonsaure and so on. (Ref.)

In acute conditions 1-3 times daily 1 ampoule (of 2ml) injected intramuscularly (im) or subcutaneously (sc). (Ref.) The vial can be administrated sublingual but is is preferred via injection, i.m. or s.c.

More about the therapy in German: http://www.windstosser-museum.info/museum/manuskript/aufklaerung/25.pdf (use google translator to translate)

Chitosan formulation:

Here is an e-mail I received from a friend from Cancer Compass (Jcancom) on a new formulation:

Dear Daniel:

An even more recent patent that you sent me was using at most 0.5mg/kg/day methylglyoxal in a chitosan encapsulation. This would be even better. The instructions they give seem easy to do. I wonder whether this formulation could be bought somewhere? https://www.google.com/patents/WO2015049689A1?cl=en

Sincerely,
Jcancom

Here are a few articles on the same MG-chitosan combination:

I will soon have a look at the encapsulation of MG with chitosan since this is probably a much better route of administration, and chitosan is available and cheap. As soon as I have news on this I will update the page.

Synergy and Antagonism:

Creatine: New paper published in 2016 from dr. Roy, suggesting Creatine as supporting MG treatment: These data strongly suggest that creatine supplementation may gain importance as a safe and effective supplement in therapeutic intervention with the anti-cancer agent MG. http://www.ncbi.nlm.nih.gov/pubmed/27138627

Glyoxalase I inhibitors: inhibition of GLO1 leads to the intracellular accumulation of methylglyoxal. The natural extracts Naringin (Ref.) and Curcumin (Ref.) are known to act as GLO1 inhibitors.

Ascorbic acidhttp://www.ncbi.nlm.nih.gov/pubmed/16112157 http://www.ncbi.nlm.nih.gov/pubmed/1995489

GABA:
One of the potential anticancer mechanisms of MG may be related to GABA A receptor. This is because MG seems to be an activator of GABA A receptor (Ref.). And GABA is known to show anti cancer activity via the GABA A receptor (Ref.1, Ref.2). Therefore, combining MG treatment with GABA supplementation may increase the chance for a successful treatment. GABA is a commercially available supplement.

Dichloroacetate (DCA):
MG triggers GABA A receptor activation which in turn triggers an influx of Cl- ions (Ref.).  Intracellular Cl- on the other hand is expected to reduce DCA’s anti cancer activity as it will reduce the efficiency of the DCA-induced GSTZ1 inactivation process (Ref.). As a result, I would not combine MG with DCA.

Update 10-March-2017: Metformin:
A great anti cancer drug, accessible and low/no side effects, but according to this article, unfortunately, Metformin may increase the Glo1 activity and as a result reduce MG effectiveness. Thus, Metformin should not be combined with MG: https://www.ncbi.nlm.nih.gov/pubmed/24710646

Safety:

Based on an anecdotal report, a patient receiving MG got seven / eight weeks after he started with the methylglyoxal high fever, (41degrees). He stopped for one week MG and started again with no other issues. (Ref.)

Overall, the potential beneficial effects of methylglyoxal far outweigh its possible toxic role in vivo, and it should be utilized for the benefit of suffering humanity. (Ref.)

When reading the patent on Methylglyoxal I found the following statement:
“Moreover, it was observed that creatine present in cardiac cells completely protected the animal from any possible deleterious effect of methylglyoxal treatment on cardiac mitochondria (Sinha Roy et al., 2003).” http://www.google.com/patents/US20110118327 Also another similar statement is here: “Furthermore, creatine can protect cardiac mitochondria from the deleterious effects of some anticancer compounds” http://publicationslist.org/data/theo.wallimann/ref-253/Ray-CK_Creatine_anti-cancer.AAS-2011.pdf
Although in the clinical studies they did not used Creatine, following these statements, I would clearly make sure that (maybe 30-60 minutes) prior to each MG administration there is a Creatine supplement administer too.
.
Source:

MG preparation:
Methylglyoxal can be bought from Western or Chinese chemical suppliers under CAS number 78-98-8. It is usually found as 40% Methylglyoxal in water solution. Here is an example of a supplier: https://www.scbt.com/datasheet-250394-methylglyoxal-solution.html

MG Clinics and Hospitals:
The Indian hospital from enclosed reference (Ref.) was still running clinical trials in May 2016. Patients can access MG at the following address in India: Lokmanya Medical Research Centre, Chinchwad, Pune 411 033

It seems that a Mexican clinic at Providence Pacific Hospital administered methylglyoxal to humans at the begging of 2000 but I can not find more info on that.

Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid.

Update on 21-June-2016:

Prof Koch’s formulation:

Yesterday, I was informed by a friend from Cancer Compass (thank you Jet) that various sources of information indicate that Dr. Koch shared his homeopathic data and formulas with a Dr. Eric Reinstorff in Germany (now deceased).  But that Dr. Dieter Reinstorff in Hamburg, Germany (Eric’s son) is still manufacturing, distributing it, and using Koch’s homeopathic reagents.

Indeed, here is an interview (from 2005, in German) with Dr. Reinstorff referring to his work with Prof. Koch. As a result, he started up a small pharma company („REIKO“ Pharma Vertriebsgesellschaft GmbH, „REIKO“ comes from Reinstorff-Koch) owning 3 different products based on Koch’s formulations: Carbonylgruppen, Rhodizonsäure, Parabenzochinon. The products seems to be manufactured by Adjupharm GmbH in Germany while being advertised at the following website http://wulf-rabe.de/molekulartherapie.php. The products are available at most German pharmacies without prescription:

In USA, it seems that the above vials are available too but 10 vials would cost 300$ http://www.arrowheadhealthworks.com/KochTMT.htm

Patents:

In vivo assessment of toxicity and pharmacokinetics of methylglyoxal 

A pharmaceutical composition and treatment method to reduce the proliferation of cancerous or tumor cells, in which the combined active agents are methylglyoxal, ascorbic acid, creatine and melatonin.

Oral formulation of methylglyoxal and its imino acid conjugates for human use https://www.google.com/patents/US20030087951

The invention relates to an oral formulation of methylglyoxal and/or its imino acid conjugates for human use and methods for preparing the compositions. Particularly, the invention relates to compositions comprising methylglyoxal and more particularly, imino acid conjugates of methylglyoxal. The present invention also relates to formulations of methylglyoxal and imino acid conjugates of methylglyoxal that can be used for the treatment and suppression of malignant diseases including but not limited to the cancers of Colon, Prostate, Pancreas, Lung, Oral cavity, Glioblastoma, and Leukemia.

Sustained release formulations containing methylglyoxal and their therapeutic applications https://www.google.com/patents/WO2015049689A1?cl=en

A novel nano drug composition for the treatment of cancer comprising 0.125-0.5 mg of methylglyoxal as conjugated to nanoparticles of chitosan, its derivatives, or other polymers; 25-100 mg of ascorbic acid; 75-300 mg of creatine; and 0.125-0.5mg of melatonin, wherein all constituents are meant for each kg of body weight.

Treatment of cancer by oxidation-reduction potentiation of cancerostatic dicarbonyls https://www.google.com/patents/US8163796

A novel treatment regimen is described for the control and elimination of cancer cell populations including cancer stem cells. The disclosed protocol consists of a pretreatment step followed by a treatment step. The pretreatment step sensitizes cancer cells to apoptosis by altering their intracellular oxidation-reduction state via reduced glutathione depletion. The treatment step involves the sequential administration of a cancerostatic dicarbonyl compound to induce apoptosis. The use of nanoparticle delivery systems further enhances both the pharmacokinetic and pharmacodynamic properties of the pretreatment compounds and the cancerostatic dicarbonyls. Since the pretreatment and treatment compounds are carefully selected and delivered, normal cells are not affected and side effects are kept to a minimum.

Reference:

Protein and nucleotide damage by glyoxal and methylglyoxal in physiological systems – role in ageing and disease http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649415/

Glycation of proteins, nucleotides and basic phospholipids by glyoxal and methylglyoxal – physiological substrates of glyoxalase 1 – is potentially damaging to the proteome, genome and lipidome. Glyoxalase 1 suppresses glycation by these α-oxoaldehyde metabolites and thereby represents part of the enzymatic defence against glycation. Albert Szent-Gyorgyi pioneered and struggled to understand the physiological function of methylglyoxal and the glyoxalase system. We now appreciate glyoxalase 1 protects against dicarbonyl modifications of the proteome, genome and lipome. Latest research suggests there are functional modifications of this process – implying a role in cell signalling, ageing and disease.

Methylglyoxal enhances cisplatin-induced cytotoxicity by activating protein kinase Cdeltahttp://www.ncbi.nlm.nih.gov/pubmed/11707430/

Importantly, co-treatment of cells with the reactive carbonyl MGO and cisplatin increased apoptosis by 90% over the expected additive effect of combined MGO and cisplatin treatment. This same synergism was also observed when ROS generation was examined. MGO and cisplatin increased PKCdelta activity by 4-fold

Effects of methylglyoxal and glyoxalase I inhibition on breast cancer cells proliferation, invasion, and apoptosis through modulation of MAPKs, MMP9, and Bcl-2. http://www.ncbi.nlm.nih.gov/pubmed/26618552

Collectively, these data indicate that MG or inhibition of GLOI induces anticancer effects in breast cancer cells and that these effects are potentiated by combination of the 2.

Triple negative tumors accumulate significantly less methylglyoxal specific adducts than other human breast cancer subtypes http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170620/

Interestingly, the activity of glyoxalase 1 (Glo-1), an enzyme that detoxifies MG, was significantly higher in triple negative than in other subtype lesions, suggesting that these aggressive tumors are able to develop an efficient response against dicarbonyl stress. Using breast cancer cell lines, we substantiated these clinical observations by showing that, in contrast to triple positive, triple negative cells induced Glo-1 expression and activity in response to MG treatment.

Inactivation of glyceraldehyde-3-phosphate dehydrogenase of human malignant cells by methylglyoxal. http://www.ncbi.nlm.nih.gov/pubmed/9450641

The effect of methylglyoxal on the activity of glyceraldehyde-3-phosphate dehydrogenase (GA3PD) of several normal human tissues and benign and malignant tumors has been tested. Methylglyoxal inactivated GA3PD of all the malignant cells (47 samples) and the degree of inactivation was in the range of 25-90%, but it had no inhibitory effect on this enzyme from several normal cells (24 samples) and benign tumors (13 samples). When the effect of methylglyoxal on other two dehydrogenases namely glucose 6-phosphate dehydrogenase (G6PD) and L-lactic dehydrogenase (LDH) of similar cells was tested as controls it has been observed that methylglyoxal has some inactivating effect on G6PD of all the normal, benign and malignant samples tested, whereas, LDH remained completely unaffected. These studies indicate that the inactivating effect of methylglyoxal on GA3PD specifically of the malignant cells may be a common feature of all the malignant cells, and this phenomenon can be used as a simple and rapid device for the detection of malignancy.

Nanofabrication of methylglyoxal with chitosan biopolymer: a potential tool for enhancement of its anticancer effecthttp://www.ncbi.nlm.nih.gov/pubmed/25999714

Fourier transform infrared spectroscopy revealed the presence of imine groups in Nano-MG due to conjugation of the amino group of chitosan and carbonyl group of MG with diameters of nanoparticles ranging from 50-100 nm. The zeta potential of Nano-MG was +21 mV and they contained approximately 100 μg of MG in 1 mL of solution. In vitro studies with Nano-MG showed higher cytotoxicity and enhanced rate of apoptosis in the HBL-100 cell line in comparison with bare MG, but no detrimental effect on normal mouse myoblast cell line C2C12 at the concerned doses. Studies with EAC cells also showed increased cell death of nearly 1.5 times. Nano-MG had similar cytotoxic effects on A549 cells. In vivo studies further demonstrated the efficacy of Nano-MG over bare MG and found them to be about 400 times more potent in EAC-bearing mice and nearly 80 times more effective in sarcoma-180-bearing mice. Administration of ascorbic acid and creatine during in vivo treatments augmented the anticancer effect of Nano-MG.

Glyceraldehyde-3-phosphate dehydrogenase: a promising target for molecular therapy in hepatocellular carcinoma. http://www.ncbi.nlm.nih.gov/pubmed/22964488

Hepatocellular carcinoma (HCC) is one of the most highly lethal malignancies ranking as the third leading-cause of cancer-related death worldwide. Although surgical resection and transplantation are effective curative therapies, very few patients qualify for such treatments due to the advanced stage of the disease at diagnosis. In this context, loco-regional therapies provide a viable therapeutic alternative with minimal systemic toxicity. However, as chemoresistance and tumor recurrence negatively impact the success of therapy resulting in poorer patient outcomes it is imperative to identify new molecular target(s) in cancer cells that could be effectively targeted by novel agents. Recent research has demonstrated that proliferation in HCC is associated with increased glucose metabolism. The glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a multifunctional protein primarily recognized for its role in glucose metabolism, has already been shown to affect the proliferative potential of cancer cells. In human HCC, the increased expression of GAPDH is invariably associated with enhanced glycolytic capacity facilitating tumor progression. Though it is not yet known whether GAPDH up-regulation contributes to tumorigenesis sensu stricto, emerging evidence points to the existence of a link between GAPDH up-regulation and the promotion of survival mechanisms in cancer cells as well as chemoresistance. The involvement of GAPDH in several hepatocarcinogenic mechanisms (e.g. viral hepatitis, metabolic alterations) and its sensitivity to a new class of prospective anticancer agents prompted us to review the current understanding of the therapeutic potential of targeting GAPDH in HCC.

Anti-Cancer Strategies of Methylglyoxal http://www.ijpr.in/Data/Archives/2015/july/2006201502.pdf

Methylglyoxal a simple carbonyl compound containing a reactive aldehyde and a ketonic group which stops the growth of cancer cells without poisoning normal cells. It is also called as Retine. These are very small molecules that are highly potent in controlling cell division. This compound inhibits the enzymes required for cancer cell and infected cell to grow by respiration and does not harm normal cells. As cancer cells require large amount of energy to multiply which was provided by ATP. Methylglyoxal inactivates the enzyme Glyceraldehyde-3-phosphate Dehydrogenase (GA3PD) needed for the ATP production in cancer cells and there by starves the cell to death and normal cells remain unaffected. As it is a carbonyl group, it inhibits the mitochondrial respiration followed by Glycolysis and Kreb’s cycle which play a major role in the production of ATP and supplies the energy to infected cell up to demand. It also play a role in binding of oxygen at cellular level and preventing the proteins to desaturate and inhibits the production of free radicals. Hence suitable energy and oxygen are unavailable to cancerous cell to grow, leading to death of the cell. It was believed that “If cancer cell cannot grow, it dies by itself”. It desaturate the proteins of malignant cell at cellular level by means of its ketoaldehyde group with an aminoacid of a protein causing the death of cell

The results clearly indicate that Nano-MG may constitute a promising tool in anticancer therapeutics in the near future.

Cancerostatic Action of Methylglyoxal http://science.sciencemag.org/content/160/3832/1140

Creatine supplementation with methylglyoxal: a potent therapy for cancer in experimental models. http://www.ncbi.nlm.nih.gov/pubmed/27138627

In conclusion, it may be stated that the anti-cancer effect of MG is enhanced by concomitant creatine supplementation, both in chemically transformed (by 3MC) muscle cells in vitro as well as in sarcoma animal model in vivo. These data strongly suggest that creatine supplementation may gain importance as a safe and effective supplement in therapeutic intervention with the anti-cancer agent MG.

Curcumin inhibits glyoxalase 1: a possible link to its anti-inflammatory and anti-tumor activity. http://www.ncbi.nlm.nih.gov/pubmed/18946510

The results described herein provide new insights into curcumin‘s biological activities as they indicate that inhibition of Glo1 by curcumin may result in non-tolerable levels of MGO and GSH, which, in turn, modulate various metabolic cellular pathways including depletion of cellular ATP and GSH content. This may account for curcumin‘s potency as an anti-inflammatory and anti-tumor agent. The findings support the use of curcumin as a potential therapeutic agent.

Curcumin inhibits advanced glycation end product-induced oxidative stress and inflammatory responses in endothelial cell damage via trapping methylglyoxal http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732849/

Methylglyoxal (MGO) inhibits proliferation and induces cell death of human glioblastoma multiforme T98G and U87MG cellshttp://www.ncbi.nlm.nih.gov/pubmed/27133062

We have also revealed that MGO induces senescence of U87MG but not T98G cells, but further studies are necessary in order to clarify and check mechanism of action of methylglyoxal and it Is a positive phenomenon for the treatment of GBM.

A novel mechanism of methylglyoxal cytotoxicity in prostate cancer cells. http://www.ncbi.nlm.nih.gov/pubmed/23333621

The results suggest that this physiological compound merits investigation as a potential chemo-preventive/-therapeutic agent, in differently aggressive prostate cancers.

Disclaimer:

This site is not designed to and does not provide medical advice, professional diagnosis, opinion, treatment or services to you or to any other individual. Through this site and linkages to other sites, I provide general information for educational purposes only. The information provided in this site, or through linkages to other sites, is not a substitute for medical or professional care, and you should not use the information in place of a visit, call consultation or the advice of your physician or other healthcare provider. I am not liable or responsible for any advice, course of treatment, diagnosis or any other information, services or product you obtain through this site. This is just my own personal opinion regarding what we have learned on this road.

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Momma boy
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Momma boy

Hello,

My mother just started methylglyoxal treatment 2 days ago for end stage Gall Bladdar cancer. We are giving MG 5mldose 4 times a day in 200ml of water. Other things that we are giving her is creatine powder, VitC tabs, B-complex, Curcumin. Now we are waiting for the drug to work.

Cathy V
Guest
Cathy V

Momma Boy Did you order it yourselves?

Emad
Member
Emad

39% Complete remission ! , This sounds even better than Salinomycin !!!

I hope its hard for cancer to develop any resistant against it 🙂

Momma Boy , just like what Daniel said , keep us up to date

hope your mother will get better soon .

Meech
Member

Hi Daniel, where would one go about acquiring this and what dose/administration of the product from the supplier would you suggest?

Thanks,
Meech

Emad
Member
Emad

Daniel did you tried this treatment or not yet ?

and your experience about the MG from china , do you think its good just like the western versions ?

——————

keeping these kind of treatments on hold & away from the public are the biggest crime in history

thank you so much for sharing all this Daniel 🙂

Emad
Member
Emad

Daniel

the tumor marker for my mother raised again from 460 to 560

I don’t know why ?

the same treatment that was successful last month , now failed !!!???

the other thing is, do you know about a trusted Chinese supplier for Methylglyoxal ??

Momma boy
Guest
Momma boy

@ Emad

What treatment She is undergoing now?

Emad
Member
Emad

the tumor marker was 712
she started DCA + (5-FU & Vinorelbine) + (Hydroxychloroqiune 400mg & Lansoprazol 60mg)
after 3 weeks the markers became 670
after another cycle , 4 weeks , the markers became 460
now with the same treatment , the markers raised again to 560 !??

i didn’t hear about any cancer that developed a complete resistance against a treatment just in less than 3 weeks !!!!

is this thing happening normally ??

but there is a little difference in the last cycle :
1- she stopped taking DCA for 3 days before chemo (usually she is stopping DCA for 1 day only) , then she started with chemo
2- after another 8 days (after chemo) she started DCA with 25mg/kg for about 1 week (just like all the other cycles , but now she didn’t take Vitamin B1) , then I lowered the dose to 15mg/kg in the second week . (normally i am not lowering the dose that much)

but this is still a little difference to me , and that just changed the game !!????
i really can’t understand what is just happening , and my mother is losing faith from everything

sorry for my long comment Daniel , but what you suggest in this situation ?

do you have a good protocol (good combination of treatments) for us to use ?

thank you so much

Emad
Member
Emad

Thank you so much Daniel

and I understand very well that you can’t suggest or advice anyone to use anything , I agree with that

about DCA , my mother is feeling like numbness & burning in her legs , I’m am afraid of neuropathy , that’s why I lowered the dose but now I don’t know what to do with neuropathy is it okay to continue with it or what should I do

the other thing , we need to stop chemo as soon as possible , my mother is on chemo for 9 months , we need to change to another new protocol with strong treatments that doesn’t cause any bad side effects , MG will be the first , but even with a strong treatment like MG , I need to add more weapons to our arsenal

for example : how about this combination : MG + DCA + Mebendazol + Griseofulvin + Artemisinin

in your opinion is this is a strong protocol ? or still need to add more ??

sorry for my repeated long questions , God bless you for helping my mother

Momma boy
Guest
Momma boy

Dear Daniel, Can you help me out bro. My Mum is dying with Stage 4 GB cancer. Can you recommend some alternative drugs(in the link below) which She can take to halt tumour growth? Sorry if I sound desperate or stupid. 🙁 Onco has given her 2-3 months to live. 🙁

http://projects.propublica.org/graphics/cancer-drug-alternatives

Emad
Member
Emad

Momma boy what about MG ?

isn’t it effective ? how did you know that its not effective with your mother ?

the other thing is looking for a clinic but they will cost a lot 🙁

i hope your mother get better soon

Fred
Member
Fred

Daniel, from http://www.williamfkoch.com/web/version2/biography.php this Dr. Koch statement is interesting:

“… If the patient has been starved a long time because of cancer of the stomach, a large cancer, paradoxical as it is, is a benefit since, once killed, it supplies food elements and actually gives strength to the patient.”

Is this somehow beneficial TLS?

Also, he describes someone starved because of cancer of the stomach. This gets near to my wife’s condition as her ability to ingest food/water diminishes as weeks go by. While she may be able to take oral methylglyoxal at this time I must look forward to an alternate administration:

From Dr. Koch:

“… This substance when purified, taken up in water and immediately injected subcutaneously into a cancer patient, causes practically no local reaction; but instead, after about 24 hours, a very decided focal reaction takes place. Wherever the cancer tissue may be, its’ cells are killed, their ionic concentration increases, the osmotic pressure increases, they take up water, swell and disintegrate. The swelling causes pain and the absorbed, disintegrated products are oxidized causing fever.”

Since MG is water soluble is there an IV equivalent that is safe?

Fred
Member
Fred

There was mention in Dr. Koch’s writings of intraperitoneal and subcutaneous injections with quick results. I wonder why current administration is oral.

Paul
Guest
Paul

Hello everyone, great work Daniel. I wonder how we can address methylglyoxal´s bad behavior of depleting good HDL cholesterol
http://www.science20.com/news_articles/sugar_substance_methylglyoxal_damages_good_hdl_cholesterol_study-143828 causing heart problems. Prof. Ray lost three patients of 46 due to heart attacks and two other ones for unclear reasons not related to cancer. http://www.cancer-therapy.org/CT/v4/B/HTML/17.%20Talukdar%20et%20al,%20205-222.html

Paul
Guest
Paul

TACE does not seem to be an option in her case maybe SIR spheres in the future. The problem is that only about 40% of her tumors show up on PET CT so I need to investigate further Salinomycin, 3BP, Diflusinal. We started DCA a week ago but I’ve just discovered a dark side of it:
https://www.researchgate.net/publication/44663282_Sodium_dichloroacetate_DCA_reduces_apoptosis_in_colorectal_tumor_hypoxia
Thinking about turning hypoxic cells into normoxi ones to get easier rid of them. What do you think?

Paul
Guest
Paul

It is difference between the spots. The largest one covering the womb is dark grey two others close to it are bright white and in the liver and around it is a mix of dark and bright spots.

Paul
Guest
Paul

Thank you Daniel. We are already using Metformin will check the rest if they are purchasable for a somewhat human price preferably in Europe. I stumbled upon this article about MG http://www.sciencedirect.com/science/article/pii/S092544391100072X difficult to interpret but it looks like that MG maybe is not suitable to treat colorectal cancer. What do you think?

Meech
Member

Something interesting that I’ve found is that ketogenic diets can increase the amount of Methylglyoxal within the body naturally. It could add to why ketogenic diets have shown efficacy.

Paul
Guest
Paul

It is a little bit confusing the Japanese researchers stated that MG is a metabolism product from glucose in the body. So the more sugar you eat the more MG. http://www.sciencedirect.com/science/article/pii/S092544391100072X
Could it be so that cancer cells have difficulties to adopt to extreme levels of sugar both low and high? I have something in the back of my head about “Mountain Dew effect” from Cancer Compass, I think.
In short a guy was drinking a lot of Mountain Dew during his anti cancer treatment and got very good results if I remember well. Could it be the effect of MG or just sugar awakes sleeping cancer cells and this way they can be killed?

Emad
Member
Emad

Hello Daniel

our friend Jcancom wrote this about MG :

———————
One thing to point out to you and others on the thread was that we had a slight trouble earlier with the dosing of MG. There was a substantial overdosing with it. Be careful not to confuse % by weight/volume for a molarity. I have no idea why they would label the way that they do.

Sigma’s 40% MG solution is not a molarity measurement
https://www.cancercompass.com/message-board/message/all,65701,360.htm
————————

so what is your opinion ?

the second thing , are you willing to try MG IV ?

we are about to receive our first MG and Salinomycin , I will love to administrate MG as IV if it’s safer and better

the last thing , the Vitamin C , should it be chewable ? not normal tablet or capsule ?

we have Liposomal vitamin C , I’m not sure if it will do the job

Hanh Nguyen
Guest
Hanh Nguyen

Hello Danielle, I am new to this. My husband is stage 4 colon cancer and it is progressing after Folfox, folfiri. Now his oncologist put him on Avastin and 5-FU. However, I do not have much faith. He ‘s been on cimetidine and metformin. I recently add Artemisinin. I would like to add MG but does he have to stop chemo or he can do these concurrently with chemo. I know you do not give advice but I appreciate your knowledge and I just need to know if people do these treatments alone or in combination with chemo.
Thank you so much.
Hanh

Hanh Nguyen
Guest
Hanh Nguyen

Also how can you buy from the supplier. I tried and they said they only sell to research facility or university.
Hanh

Hanh Nguyen
Guest
Hanh Nguyen

I just recognize that I misspell your name. I am really sorry but since the new of my husband’s cancer progression I could not think straight. Just the thought that he won’t be here for long is killing me. I’ve been crying so much for the last few days that makes reading so hard to understand and remember.
Again, my apology.
Hanh

Mike
Guest
Mike

Are the methylglyoxal and koch treatments designed to be used separately or simultaneously?

Dr. Michael
Guest
Dr. Michael

I have researched and studied methylglyoxal as a cancerostatic agent for 40 years. Dr. Szent-Gyorgyi, with whom I occasionally corresponded in the 70’s and 80’s, was an incredible visionary. He recognized the uniqueness of methylglyoxal and how it could quite selectively kill cancer cells while not harming normal cells at millimolar concentrations. However, he also understood the challenge of delivering a pharmaceutically active concentration to the tumor site in the face of the high glyoxalase I enzyme levels in cancer cells. Interest in methylglyoxal as a cancer treatment has increased in the past 20 years. People have looked at ways to inhibit glyoxalase I activity with specific inhibitors as well as protect methylglyoxal prior to delivery to the cancer site with nanoparticles and more recently Dr. Ray with chitosan. I have tried to encapsulate methylglyoxal with 60-100nm particles unsuccessfully. While some of these approaches have been successful in increasing methylglyoxal effectiveness their still not good enough leaving 20% viability and perhaps inducing drug resistance. As far as IV delivery, methylglyoxal is very acidic, even when diluted with pH typically 5.5 or less. Adjusting the pH up tips the equilibrium from the active keto form to the hydrates. My approach which will be tested shortly(this year) regulates the cancer cell redox state making the treatment with methylglyoxal far more efficacious ( 0% viability) @ 100micromolar. I hope this sheds some additional light on the discussion.

Dr. Michael
Guest
Dr. Michael

Dear Daniel:
1. I am looking at MG doses that would be 20-50% of Dr. Ray’s. When administered orally, Dr. Ray typically gives 30mg/kg body weight /day.
2. Testing initially will not be on humans. However the test system is very new, innovative and quick turn times. Initial testing will be done on triple negative breast cancer cells due to the current lack of success for standard therapies.
3. I don’t have a strong opinion on heart related methylglyoxal toxicity.

Emad
Member
Emad

Hello Daniel

hope you and your wife are doing good 🙂

I like to share a little bit about what happened last 2 months

the tumor marker was 939 , but when we test again it was 712 not 939

and it raised from 450 to 712 because of stopping both chemo and DCA

the oncologist decided to change to another chemo : (carboplatin + gemzar)

because of neuropathy I didn’t try DCA orally

after 3 weeks on chemo only , there was a very little decline in tumor marker , from 712 to 685

then after my mother felt good (less neuropathy) I decided to give her DCA again , but now I started on IV’s

but I started with low doses and raise gradually , 1 or 2 IVs per week , 45mg/KG

after 3 weeks (chemo + low dose DCA) the tumor marker raised to 699 !!!

I felt so bad that time and confused , but I thought maybe it is still early for DCA to work !?

or maybe the cancer became resistant to both DCA and Chemo together !!?

I started to give my mother higher doses of DCA

2 to 3 times per week , 70mg/KG each IV

after 3 weeks (today) the result is : tumor marker declined from 699 to 517

I was so happy that the DCA and Chemo combination are still working , and the good thing about that is the side effects are very low with DCA IV , there is neuropathy but not as bad as when taking DCA orally

and the other thing , this chemo drug (carboplatin + gemzar) have a little side effects on my mother

———–

I shouldn’t forget , finally Methylglyoxal and Salinomycin arrived to Libya

tomorrow we gonna receive them , and I also have creatine and chewable vitamin C

but I’m not sure how to start with it , should I combine MG with DCA and Chemo ? or should cancel DCA ? or maybe keep them all and just raise MG doses gradually ?

I’m not sure what to do

and regarding Salinomycin , I don’t have Kolliphor , Sigma are not responding at all

I could not find Kolliphor anywhere other than Sigma , and the only choices I have is ethanol or DMSO

I don’t know if its okay to use one of them , but maybe it’s better than just waiting …

that’s all my story to now , thank you so much

my best wishes for you and all the other fighters around the world 🙂

Emad
Member
Emad

its stored in the freezer right not for sure

i meant : its stored in the freezer right not for sure

(mistype)

Emad
Member
Emad

its stored in the freezer right NOW for sure

i keep typing it wrong , my mistake

anna
Member

As you maybe already read about our case we have a lot of issues with liver due to unsuccesful SIRT treatment.
I was planning to use methylglyoxal – but then I found this http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206746/ .
Any advice?

oknab9
Member

Hello. I hope you can help me with your advice. A 75 year old relative of mine has been diagnosed with stage 2 non-Hodgkin lymphoma of the stomach and pelvis. He’s also had Parkinson’s disease since 2010, and two bypass operations of the heart. I’m thinking about getting him on the Koch program of:
Rhodizonsäure, Parabenzochinon and Carbonylgruppen from Adjupharm. Do you think that is a good idea? Can he take these together with medication he’s taking now? Do you have any other suggestions maybe?
Thank you

Ergin
Member
Ergin

Hi Friends,
I wonder if any of our friends is now on MG or tried it before?
I want to begin MG as soon as possible with your experiences if you have.
Do you have any idea where to buy it.?
From buying Sigma is very hard because it takes 6 weeks.

I have found this article about the toxicity about MG.I liked it.%80 of animals cured.

http://www.sciencedirect.com/science/article/pii/S0041008X0500400X

Kind Regards
Ergin

Ergin
Member
Ergin

Dear Daniel,
Again thank you for your kind responds.Sorry for too many questions.
I hope chemo will work.I am thinking for surgery too much these days.But if chemo will not work?a surgery with hopeless.
I took too much responsibility and went to a wrong doctor,it makes me very very sad in each day.
We choose the wrong way,we must go to surgery when ca125 declined to 5 after first 3 cycles of chemo.
Thinking of salinomycin but i couldnt find a dr to use it.
But we will try all posibilities and find a way to chemo work again.
I believe this.
Kind Regards
Ergin

Alex
Member

Great. wasted a full hour typing here……. only to get a message saying i am trying to spam ffssssssssss

Please someone contact me.

I want to get and share information/experience.
I prefer skype for this.
Got some stuff here with Graviola, Paw Paw, Aspirin, CBD, Ginger.
And many more. if only my comment wouldn’t have been canceled by the spam filter. i would have shared it all here.

Please contact me.

May you have good health and fortune.
Snake

Alex
Member

Anyone with experience on Paw Paw, Graviola?
Anyone tried aspirin?

I’m getting some good results with them.
Also, CBD oil and Ginger amongs others

I’m looking to exchange experience and information.
Please contact me, read the above post.

Alex
Member

Doing 10g/graviola leaf powder infusion (tea) every 4 hours
2 paw paw capsules 3 times a day
about 30 drops of the oil mentioned bellow *** on ocasion now.

wondering what else to add on top of this protocol
Available things:
– artemisinin
-hemp 90% cbd 10% oil ***
-betadine
-Aspirin
-Vitamin D
-COD liver oil
-Flax seed oil
-olive oil
-coconut oil
-Vitamin C
-Curcumin
-Ginger
-Appricot kernels
-nistatin
-iv vitamin C
-multi vitamin from organic source
-Propolis
-pumpkin seeds

some more medicine…… a full bag of tricks.
i don’t wanna brag or complain but my room smells greenish – funny ha?

I want to get the most out of what i have around for now.

I’m fairly convinced that it’s not just items in the protocol that contribute to healing but also a good strategy based on science and personal experience.

SO… here to learn.

got some people suggesting and promoting the following as “the cure”:
black seed oil
salvestrol/resveratrol
I’m quite sceptical tho.

Looking forward for anyone’s opinion or reaction.
Thank you very much Daniel!!!

Helga
Member

Dear Alex,

I have read your story, sorry to hear that your mom is not doing so well. I agree, surgery may not be the best option for a metastasized tumor. The reason is that the tumor itself secretes anticancer proteins to suppress the growth of the other tumors. They were isolated by Judah Folkman in 1999. Once the main tumor is removed, this inhibitory effect goes with it and metastatic tumors will grow more easily.

Anyway, regarding your treatments are not sufficient I think. Paw-paw was proven to arrest growth by preventing fuel from food to reach the tumors. So it is good to take around meals I think.

Have you tried to inhale Lapacho tea? It can be quite effective against lung ailments. And also, would you consider citric acid? As your mom has not received chemo yet, it might work esp. well for her. I am taking now 12 grams per day in 6 doses. I feel that it is important to spread it out during the day (I suspect I have ovarian cancer). It makes me feel so much better, amazing. But it is important to keep the body alkalinized. There is tremendous valuable information on Daniel’s site in general and also on citric acid. I myself wrote about an article written in 1866 (!) about the use of CA to treat cancer in a respectable medical journal. I am not sure if CA interferes with paw paw, you could always do some internet search yourself.

Also enzyme therapy is great for cancer. If your mom’s heart is strong, you could try oleander treatment. If you are cash strapped, you could make your own, although Ergin is against it. But if you follow the instructions closely, it is safe. I myself cooked it once and then used to take it. The important thing is to filter the “tea” twice, very carefully, to make sure that no pieces of the leaves are left in the final product. It is described somewhere how to make it exactly.

Beta glucan is great plus you might consider cimetidine. Also, if she can tolerate heat, an infrasauna would be a good idea. Best thing would be for you to read this site carefully, it is a treasure trove of useful information! And I have come across hundreds of sites about “alternative” treatments of cancer. Daniel’s site uniquely combines the best of both worlds. Just read his articles.

Kind regards,
Helga

alternmed
Member

hi helga

can you provide the recipe for oleander? as i m trying to find a prepared one,but no luck until today

thanks

Helga
Member

hi,

this is the site I used: http://www.tbyil.com/oleandersoup.htm
The filtering using 4 coffee filters etc are the most important part, you must observe it. What cancer do you deal with, may I ask?

Alex
Member

Dear friends,

First of all Thank You!!!
For reading, for tyring to help.
I don’t even know where to start from. It’s going to be a long one, sorry!
Markers almost doubled in 20 days of paw paw / graviola treatment
We did aspirin / CBD oil before for a couple of weeks, and that seemed to work better, no numbers to prove it but the CEA level was 28.05 just about week after switching to graviola
Now the CEA level is 43+, no exact number yet, Also CA 19-9 dropped a few points from what i heard. They were bellow the refrence value in the begining and now even lower….
It’s funny because since her pain was gone this entire time, we were expecting a drop. Apparently pain absence may not be an indicative of healing.

I am wondering weather the CEA levels are higher because of other crazy reasons such as tumour necrosis, some liver problems etc……….???????
This is when i wish i had a CT scan room next to my bedroom. Or something way better Star Trek technology, something….

It’s amaizing how little has been done in this quest compared to other problems such as, how to get a better duck lips selfie.
Today’s transistors are small enough for maybe 100 of them to be fitted with ease inside a human red blood cell if not more.
We search and find other planets that we will probably never ever reach while here we still search for solutions to some of the oldest problems in the entire known history of earth and humanity.

Everything seems so uncertain and unclear now, how do we measure anything in this world with more accuracy and certainty?
Confusion, desperation, no clear path and broken.
I am now financially stuck, only managed to pay for basic food and some bills.
I gave all my money that i had saved for a brighter future to this cause, and i don’t see the light.
I feel i have talent, ambition and tenacity to help my mother, but i need experience. Certainty and guidance.
I am feeling the ground going away from my feet as time goes, i had very high hopes in the Graviola/Paw Paw protocol since soooo many state they got well.
I don’t know how much time we have left to experiment with treatments, i need a clear certain solution that will work for sure.
I may be asking for too much, being broke, unable to do more testing, gene sequencing, more blood tests, urine etc.
I hate to leave my results to chance, but it looks like i have no choice, forced by the laws of the universe, i don’t know anymore.
Not here to complain, i realize everyone must be going trough similar issues and emotions.
So after hearing about this level of CEA, we decided to halt treatment and revisit the options.
Went ahead and told my mom to drink ginger + lemon tea, high concentrations.
Gave her a few aspirins and cbd oil, she started feeling better, no more pain, just a sense of inflamation in the area where the primary tumour was/is (spine), some burning sensations in her right breast, lymph nodes seem more proeminent on her arm.
Some stabbing pain as Ergin described with his mom.
We just did a saline wash, then a coffee enema in the evening.
Laying the foundations for a new treatment strategy begining with detox, if any is strongly suggested based on personal experience.
Please let me know what is actually working for you.
I kept looking again and again on budwick, gherson. IDK anymore what to believe…..
For some it worked maybe, for others it didn’t. What to do from here?
I am thinking about beta glucan and maybe some other things if i can find the money to bring them over from another country.
Things that Daniel suggested.

Even if i had all the possible solutions materialized before me for free. I would still be confronted with the problem of choice since time in these situations is a big problem i feel.

Helga dear, inhalation is probably next to useless in our situation since the main tumour was partially removed with the upper right part of the lung, leaving small parts embedded into her spine. (that was then) Oct 2016.
Citric acid??? did not try. Only some lemon juice. Please more information needed.
If you believe you have some cancer of sorts, i think you should go check it out with contrast substance, scanners are bad for you but knowing something for certain is very important i believe.
What about ensyme therapy? Oleander? Any certaininty? My mom has heart problems that are worse now with part of the lung missing after a extreme surgerry. Oh how wrong we were…… what a mistake, just after surgerry we said, HELL NO to the rest of the “treatment”
Compared to anyone else i know that has cancer, my mom is feeling better than some “healthy” people her age without “treatment”
She can not tolerate heat sadly, cancer doesn’t come to the healthy ones. All those kids who have cancer either have their genetics wrong or their diet change could fix it and then you would see them getting their websites, asking for hundreds of dollars to tell you their secret, healthy food! OMG the industry is keeping it secret from us.

I get nudged from left and right by people who claim miracle results from RESVERATROL and Black Seed OIL
After trying a few miracle “pills”, i can safely say…….. i’m tiered of Snake oil.
People call me Snake, i think i should get some aparatus and make / sell oil myself. It will be branded so you know it’s the original that can CURE you for sure.

Anyway, our experience points to aspiring + cbd oil as being AWESOME.
Alone? nope….
With the only problem beying the bleeding, I am pondering mixing it with vitamin K to reduce that side effect as Al was talking about on another page here.
Any opinion is welcomed.

What about Rigvir??? They seem confused too. No guarantees, no repetable precision results. This doesn’t look like science anymore.
Everyone charges what you never had your entire life. Everything is so expensive and the problem is so common. So paradoxal.

I’ve been reading here, almost everything is written with inhibited emotions behind the keyboard.
I know you all suffer so much, just as i am. We fight, more or less together to save our loved ones or ourselfs.
I didn’t cry much till i saw how many more are suffering, and seeing some fighters here keeps me a little more encouraged, with some sholder to cry on that understands my pain of seeing my mother seeking help in my eyes with her gaze.
This diagnostic “cancer” is the worst of all emotionaly because of no known actual cure for all of them, pointing to either a lack of understanding, application of knoledge and science, science itsef missing…. something is not right.
Personalization of treatment is TRUMP expensive. Society still values money more than life.

Please advise.
If anyone else is interested in talking, i’m almost always available on skype. Snake_Systems
Daniel please, if any time available. Do login.

All my best wishes to you all, may good health and happyness be with you!
Alex

Alex
Member

not again….. this comment approval thing.

Alex
Member

Dear friends,

First of all Thank You!!!
For reading, for tyring to help.
I don’t even know where to start from. It’s going to be a long one, sorry!
Markers almost doubled in 20 days of paw paw / graviola treatment
We did aspirin / CBD oil before for a couple of weeks, and that seemed to work better, no numbers to prove it but the CEA level was 28.05 just about week after switching to graviola
Now the CEA level is 43+, no exact number yet, Also CA 19-9 dropped a few points from what i heard. They were bellow the refrence value in the begining and now even lower….
It’s funny because since her pain was gone this entire time, we were expecting a drop. Apparently pain absence may not be an indicative of healing.

I am wondering weather the CEA levels are higher because of other crazy reasons such as tumour necrosis, some liver problems etc……….???????
This is when i wish i had a CT scan room next to my bedroom. Or something way better Star Trek technology, something….

It’s amaizing how little has been done in this quest compared to other problems such as, how to get a better duck lips selfie.
Today’s transistors are small enough for maybe 100 of them to be fitted with ease inside a human red blood cell if not more.
We search and find other planets that we will probably never ever reach while here we still search for solutions to some of the oldest problems in the entire known history of earth and humanity.

Alex
Member

Everything seems so uncertain and unclear now, how do we measure anything in this world with more accuracy and certainty?
Confusion, desperation, no clear path and broken.
I am now financially stuck, only managed to pay for basic food and some bills.
I gave all my money that i had saved for a brighter future to this cause, and i don’t see the light.
I feel i have talent, ambition and tenacity to help my mother, but i need experience. Certainty and guidance.
I am feeling the ground going away from my feet as time goes, i had very high hopes in the Graviola/Paw Paw protocol since soooo many state they got well.
I don’t know how much time we have left to experiment with treatments, i need a clear certain solution that will work for sure.
I may be asking for too much, being broke, unable to do more testing, gene sequencing, more blood tests, urine etc.
I hate to leave my results to chance, but it looks like i have no choice, forced by the laws of the universe, i don’t know anymore.

Alex
Member

i give up……. the webpage isn’t letting me complete the comment i wanted to post here……………….

Helga
Member

Dear Alex,

just see my comment to Paul (scrolling down to the bottom of the page you’ll find the latest posts). I wrote about wheatgrass juice treatment and how a 75 yo woman with metastatic cancer was cured by it. I feel that you lost hope, which outlook should be changed if you want your mom to be healed. Read inspiring stories on the internet, there are plenty of them. Citric acid treatment is great. For ginger, I’d recommend dried ginger instead of just ginger tea, it is so much more effective! Fresh ginger may be even better but takes more to get it. Wheatgrass, citric acid and ginger cost almost nothing. Why don’t you read here the appropriate section about citric acid. And keep up your spirit! How is you mom’s? The most important ingredient is the resolve to want to live!

Kind regards,
Helga

Matthew Shelton
Guest

Hi everyone

Daniel, thanks for your work and expertise in maintaining this wonderful site.
I am an integrative GP working with cancer patients, mainly around therapeutic nutrition, herbals, and some pharmaceuticals, cimetidine, metformin, vit D etc. I am trying to find a source of MG, but this is proving much harder than I had hoped!
No replies to emails to India, Chinese manufacturers etc.
I got a small amount off ebay that I’m certain has helped a glioma patient to some degree, but that source has disappeared.
Can anyone help??
Thanks
Matt