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Carbogen inhalation opens the blood-brain barrier without causing long-term metabolic or neurological deficit

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medline
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Abstract

The blood-brain barrier (BBB) prevents many drugs from entering the brain. Yet, conventional methods that open the BBB are technically demanding, poorly reversible, and can be associated with long-term adverse effects. In comparison, carbogen, which is introduced nearly a century ago as a treatment for psychiatric disorders, is easy to administer and readily available to many labs and hospitals. Here, we show that carbogen inhalation opened the BBB in rats, as indicated by the extravasation of an intravenous protein tracer. When the tracer was injected immediately or hours after carbogen inhalation, less tracer was detected in the rat brains, suggesting at least partial reversibility of this response after carbogen exhalation. Despite marked increase in BBB permeability, inhalation of carbogen for 30-90 min had no acute effect on the level of neuroinflammation or apoptosis in the brain, and had no long-term effect on body weight, food intake, locomotor activity, or learning and memory performance. Our study demonstrated that carbogen inhalation is a safe method to open the BBB.

https://pubmed.ncbi.nlm.nih.gov/31276640/

Carbogen is a mixture of CO2 and O2 gas.
https://en.wikipedia.org/wiki/Carbogen


   
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Jcancom
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medline, good one!

 

Here's what the wiki article you linked noted:

"When carbogen is inhaled, the increased level of carbon dioxide causes a perception, both psychological and physiological, of suffocation because the brain interprets an increase in blood carbon dioxide as a decrease in oxygen level,[3] which would generally be the case under natural circumstances. Inhalation of carbogen causes the body to react as if it were not receiving sufficient oxygen: breathing quickens and deepens, heart rate increases, and cells release alkaline buffering agents to remove carbonic acid from the bloodstream."

Wow! Cells can release alkaline buffering agents? I was unaware of this! Changing the acid/base balance of cancer cells could be of considerable relevance.

I have read up on the use of other gases such as hydrogen and nitrogen that can have a "narcosis" effect.

"Hydrogen narcosis (also known as the hydrogen effect) is the psychotropic state induced by breathing hydrogen at high pressures. Hydrogen narcosis produces symptoms such as hallucinations, disorientation, and confusion, which are similar to hallucinogenic drugs. It can be experienced by deep-sea divers who dive to 300 m (1,000 ft) below sea level breathing hydrogen mixtures.[1] However, hydrogen has far less narcotic effect than nitrogen (which causes the better known nitrogen narcosis) and is very rarely used in diving." also wiki

This is very interesting! I am not sure whether the hydrogen thread has considered this as a possibility.

Breathing high pressure Hydrogen gas? What effect would that have on cancer?

I have read online about a cancer patient with terminal uterine cancer who apparently had a rapid tumor response when diving at depth with scuba gases. This is a very exciting development!   

 

 

 


   
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johan
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johan
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though I think IOR and ICR are more protective than actually therapeutic


   
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Jcancom
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Thank you johan for the reference!

Wow! It looks like hyperbaric hydrogen could be of value. All of the treated mice had their skin tumors turn black over the course of one week of treatment! The article does not seem to make a direct comparison with normobaric hydrogen, though what the article is reporting seems beyond the results that we have seen so far with hydrogen therapy. What is of interest here is that perhaps when a chamber were to be filled with hydrogen at pressure, it might not require a great deal of additional hydrogen generation. This might allow for the same high performance machines that some of those on forum already have to be used with hyperbaric hydrogen.

Note: The pressure and mixture of hydrogen and oxygen gas used in the treatment was chemically unable to undergo combustion. 

Hyperbaric Hydrogen Therapy: A Possible Treatment for Cancer

https://docksci.com/queue/hyperbaric-hydrogen-therapy-a-possible-treatment-for-cancer_5e586c6b097c475b748b4569.html


   
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medline
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Thanks John and Jcancom!

Carbogen, breath-holding techniques (buteyko, yoga pranayama), breathing into a paper bag, natural inhibition of carbonic anhydrase enzymes (capsaicin/chili peppers, punicalin/pomegranates), etc. may help treat cancer.

Targeting Hypoxia: Carbogen Breathing

The most relevant approach to overcome tumour hypoxia is to promote oxygen delivery. Hyperbaric oxygen (HBO) treatment consists of breathing 100% oxygen at 2–4 times the normal atmospheric pressure, which results in saturated haemoglobin and increased oxygen in the circulation [5,6,7]. While HBO is applied during or briefly before the radiation treatment, multiple clinical studies, including multi-centre randomised trials by the medical research council (MRC) in late 1970s and mid-1980s, showed that HBO treatment improved local control in patients with head and neck squamous cell carcinoma (HNSCC) or cervical cancer, compared to normal air. However, technical challenges derived from the decompression of the hyperbaric chamber, due to the radiation equipment and severe tissue damage, limited the use of HBO in radiation treatment [8]. Thus, carbogen breathing (95% oxygen + 5% carbon dioxide) was suggested as an alternative to HBO as it could promote oxygen delivery due to vasodilation and increased blood flow induced by carbon dioxide [9,10]. Nonetheless, carbogen breathing showed mixed results, which could be attributed to inconsistencies in the amounts of time patients were subjected to carbogen breathing [11,12]. Although carbogen treatment was well-tolerated by paediatric patients with high-risk brain stem glioma, no efficacy was found when combined with radiation [9].

Nicotinamide is a vitamin B3-derived molecule and its initial radiosensitising effect was thought to be mediated by the inhibition of the DNA damage repair (DDR) pathway specifically by blocking poly(ADP-ribose) polymerase 1 (PARP1), which is responsible for nucleotide excision repair (BER) [10]. The combination of carbogen with nicotinamide seems to target both acute and chronic hypoxia. Further studies suggested that nicotinamide could reduce acute hypoxia by inhibiting intermittent vascular shut down [11,12]. Accelerated radiotherapy with carbogen and nicotinamide (ARCON) trials showed a better patient survival and outcome in bladder and laryngeal cancer [13,14,15,16]. Although ARCON trials indicated the effectiveness of carbogen breathing as an adjuvant treatment regimen, it is necessary to consider other studies questioning the use of carbon dioxide in patients and the effectiveness of carbogen or the combination of carbogen and nicotinamide when compared to HBO [17,18].

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615589

Achilles Heel Of Cancer Discovered – Its Lack Of CO2

Several years ago I posted about a study demonstrating that an Achilles heel of cancer had been found – i.e. its addiction to fat (not glucose, as doctors and media continue to mindlessly parrot).

https://www.eurekalert.org/news-releases/805681

As my readers know from my other posts and various podcasts, excessive fatty acid oxidation (FAO) translates into lower CO2 levels due to the fact that FAO produces less CO2 per unit of substrate (fat or glucose) oxidized, while also producing a lot more reactive oxygen species (ROS). The lower production of CO2 results in relative hypoxia inside the tumor compared to the rest of the organism and that further accelerates tumor growth. Since CO2 and lactic acid are inversely correlated, lower CO2 means higher lactate levels inside the tumor and that means more angiogenesis (lactate is the most potent endogenous stimulator of VEGF) and more tumor growth. A new study below now demonstrates that the low CO2 levels are not just a minor side effect of tumor metabolism (i.e. excessive FAO) but in fact another crucial “defense” mechanism that tumor cells use to grow and metastasize. Namely, the study found that tumors overexpress the enzyme carbonic anhydrase (specifically isoenzyme CAIX), and that inhibiting CAIX fully stopped cancer growth. In fact, the results from the animal experiments were so promising that there is an ongoing Phase I human trial for patients with pancreatic cancer using the same patented CAIX inhibitor (SLC-0111) the study below discusses. So, it does look like combining FAO inhibitors and CA inhibitors may be viable interventions for cancer that may have truly curative effects. Interestingly enough, the study also found that increased expression of CAIX prevented a type of apoptosis process in cancer cells known as ferroptosis. This is an apoptosis is driven by iron accumulation inside the cells, and it may explain the presence of so-called “anemia of chronic disease” (actually a condition of intracellular iron overload) in most cancer patients. It looks like the high ferritin levels (and thus high intracellular levels of iron) seen in this condition are an adaptive response by weakened/sick cells through which they attempt to commit apoptosis and prevent further damage to the organism. However, the lack of CO2 through the overexpression of CAIX prevents that process from achieving its goal, which not only allows the tumor to survive but also further weakens and sickens the patient due to the chronically elevated intracellular levels of iron.

A quick note on the specific drug used in the study. It is a new, patented chemical targeting specifically the CAIX isoenzyme. As such, its long term safety is unknown and the cost of using it will probably be prohibitive for most people unless it is prescribed by a doctor. In addition, there is plenty of evidence that tumors overexpress multiple CA isoenzymes, which means using a drug selective for just isoenzyme IX is likely to be less therapeutic compared to a drug that targets most/all of them. One such broad-spectrum CA inhibitor is acetazolamide, and it is a drug with decades of usage behind its back, lacking major side effects. So, the available evidence suggests that using acetazolamide would be preferable to the new, patented drug used in the study below. Moreover, recent studies have discovered that vitamin B1 (thiamine) is also a powerful CA inhibitor, on multiple CA isoenzymes, with overall effects and potency very similar to acetazolamide.

https://pubmed.ncbi.nlm.nih.gov/22145674/

Thus, using thiamine in comparable daily doses (1,000mg-1,500mg) to acetazolamide should be able to achieve the same effects with even less risk of side effects. Since thiamine also activaves PDH while also inhibiting PDK, further cements its potential as a therapy for cancer. As far as the FAO aspect of cancer metabolism, niacinamide and aspirin are perhaps the best-known and least risky options to limit both availability (through lipolysis) and oxidation of lipids by the “cancer” cells. Multiple animal studies have demonstrated robust anti-cancer effects of niacinamide in HED of 3,000mg-5,000mg daily and aspirin in the same dose range. Since vitamin B1 (thiamine) and niacinamide have synergistic effects, it seems reasonable to propose vitamin B1+B3 combination (in the dose ranges discussed above) as a generic/systemic intervention for cancer regardless of its type/location, and adding aspirin to that combination (even at lower doses than discussed above) would likely potentiate the anti-cancer effects of the vitamin combination even more.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7323835/

http://cancerimmunolres.aacrjournals.org/content/early/2019/05/14/2326-6066.CIR-18-0657.full-text.pdf

https://www.clinicaltrials.gov/ct2/show/NCT03450018?term=SLC-0111&draw=2&rank=1

http://dx.doi.org/10.1158/1538-7445.AM2021-2015

https://www.walesonline.co.uk/news/uk-news/cancer-treatment-breakthrough-scientists-discover-21427402

“…Cancer’s ‘Achilles’ Heel’ has been identified by scientists. The protein fuels tumours when oxygen levels are low, say scientists. It enables them to adapt and survive – and become more aggressive. The enzyme called CAIX (Carbonic Anhydrase IX) helps diseased cells spread to other organs. It could hold the key to new treatments for the deadliest forms including breast, pancreatic, lungs, bowel and prostate cancers. Study senior author Professor Shoukat Dedhar, of the University of British Columbia in Canada, said: “Cancer cells depend on the CAIX enzyme to survive, which ultimately makes it their ‘Achilles’ heel.’ “By inhibiting its activity, we can effectively stop the cells from growing.”

“…As the tumours advance, the blood vessels are unable to provide enough to every part. Over time, the low-oxygen environment leads to a buildup of acid inside the cells. They overcome the stress by unleashing proteins, or enzymes, that neutralise the acidic conditions. The process is behind spread, or metastasis, to other organs – which is what kills patients. Finding a way to prevent it is the ‘Holy Grail’ of cancer research – turning it into a chronic, rather than fatal, illness. One of the enzymes is CAIX. The Canadian team previously identified a unique compound known as SLC-0111 as a powerful inhibitor. It is currently being tested in clinical trials. Experiments in mice with breast, pancreatic and brain cancers showed its effectiveness. It suppressed tumour growth and spread although there were side effects, with other cellular properties diminished. Now the researchers have demonstrated other weaknesses in CAIX using a technique called genome-wide synthetic lethal screening. The powerful tool systematically deletes one gene at a time to determine if a cancer cell can be killed by eliminating the enzyme. Surprisingly, results pointed to an unexpected role of proteins and processes that control a form of cell death called ferroptosis. It happens when iron builds up and weakens a tumour’s metabolism and cell membranes. Dr Dedhar added: “We now know the CAIX enzyme blocks cancer cells from dying as a result of ferroptosis. “Combining inhibitors of CAIX, including SLC-0111, with compounds known to bring about ferroptosis results in catastrophic cell death and debilitates tumour growth.” A large international effort is currently underway to identify drugs that induce ferroptosis. The study is a major step forward in this quest.”

http://haidut.me/?p=1615

 


   
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johan
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great stuff!  Also, Rosmarinic acid (Rosemary) could help with carbonic anhydrase inhibition.


   
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Jcancom
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@johan, I do not want to bother you, though I have been having asthmatic type problems for quite a while now and I wondered whether you might have any suggestions. I have a spirometer and I have been blowing ~440 for quite some time. I would really like to normalize this and move it more towards 550, though I am unsure how to do this. I saw a doctor about this and all they could think of was the asthma inhaler which presumably I would need to use chronically forever. Whenever I try to exhale I can really feel the resistance force that is stopping the outflow. Any suggestions would be greatly appreciated.

Best Wishes, Jcancom


   
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johan
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@jcancom sorry to hear you still have asthma issues. I remember you said it came out of nowhere, you never had any related problems before. One possibility is that you've become allergic to something in your environment or something you're eating or drinking. Allergies can just come out of the blue, you reach a threshold and then the symptoms appear. Or it could be toxicity. In any case, it's causing inflammation.

Here are some suggestions:

If you haven't done this since your asthma started, change your old mattress for a hypoallergenic mattress.

Try an anti-histamine diet.

Use both a HEPA filter + separate activated carbon filter (these are cheap). Change the filters at least every 4 months.

Take activated carbon at night.

Fresh ginger tea (antihistamine).

I had a great experience with homeopathic medicine, so I would definitely try it. I found this:

#1. Arsenic Album
A top remedy, It treats asthma because of its action on mucosal inflammation.
Symptoms - burning in chest, suffocation, asthma worse at midnight, wheezing respiration.
Homeopaths give 30 potency during treatment and 200 potency during attack in alteration with blatta orientalis 200

#2. Natrum Sulph
For patient who is very sensitive to damp weather,
Humid asthma
Asthma after cold, especially children.
Rattling cough, expectoration greenish.
Chronic asthma worse in damp weather

#3. Spongia Tosta
For asthma with a dry cough.
Along with dry cough, whistling from the chest on inspiration is noted.
Respiration is also difficult

#4. Carbo Veg & Senega
Senega is excellent for asthma in aged people.
Carbo Veg is prescribed in case of cough with burning pain in the chest

Top Specialty/Complexes Homeopathy Medicines for Asthma

#1. Adel 10 Deasth drops
German Homeopathic medicine indicated for asthma symptoms in adults and children that act on bronchial asthma, cardiac asthma, broncho spasm, bronchitis.
Contains: Ammi Visnaga 4x, Aralia Racemosa 4x, Cobaltum Nitricum 6x, Dactylopius Coccus 4x, Eriodictyon Californicum 6x, Grindelia Robusta 12x, Lactuca Virosa 4x, Phosphorus 12x.
#2. Dr.Reckeweg R 43 drops
Acts as constitutional treatment for the bronchial asthma. Treats acute form of obstructive bronchitis due to increased mucus formation and swelling up of the bronchial mucosas
Contains: Ars. Alb. D8, Belladonna D30, Bryonia D12, Carbo Veg D30, Kalium Phos. D30, Hypophysis D30, Nat Chlor, D30, Nat Sulf. D200, Veratrum D30, Yerba Santa D12.

#3. Schwabe Grindelia Pentarkan tablets
It has key ingredients like ephedra vulgaris, grindelia robusta etc that acts on allergic bronchitis and mild bronchial asthma.
Contains: Ephedra vulgaris 1x 75mg, Grindelia robusta 1x 75mg, Ipecacuanha 3x 25mg, Justicia adhatoda 1x 25mg, Lobelia inflata 3x 25mg, Excipients Q.S.

#4 Biocombination No.2 Tablets
Schusslers Biochemic Cell Salt combination for Asthma.
Contains: Magnesium phosphoricum, Natrum sulphuricum, Natrum muriaticum, Kalium phosphoricum .

Let us know how it goes J!

 

 

 

 


   
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Jcancom
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@johan, thank you very much for your suggestions! It really helped when you said at the bottom of your comment to let everyone know has it goes. Sometimes the group pressure alone to get going on something is all that is needed. In fact, I started reading online about asthma and I now have a whole bunch of ideas in addition to yours that could be of help. Many of these are surprising recognizable. The basic game plan for healthy living, asthma treatment, cancer treatment seems to be distill done to almost the same list. Vitamin D, omega-3 fatty acids etc.

What is also of interest is that I consulted my full genome readout and it reported that I have an extreme polygenic score for IL18 -- a known inflammatory chemical. There are a range of natural type remedies to bring down IL18 levels. 

Over the next few days I will try and stock up on some of the items that could help me.

Thank you again, Johan. I greatly appreciate your encouragement and suggestions.

Best Wishes, Jcancom

 


   
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johan
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@jcancom I'm confident you'll get this sorted out! 


   
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Jcancom
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@johan, sorry to trouble you, yet I am wondering about the reduced methylene blue that you are now taking (MB + AA). What I am unsure about is why the leuco form would not immediately reverse back into the MB form? What would stop this? Also, might there be some way to take the leuco form and package it in a pill form? I am not sure how one could achieve this without also reverting it back to MB. 

 

Thank you for your encouragement it really does help!


   
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johan
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@jcancom 

Aparently because of the AA/DHA, note that as mentioned in the other thread I got greenish urine this morning and in fact also in the afternoon. But I've attached a pic I just took from the 25mg bottle that can be seen here (at the bottom of the page). After vigorous shaking, it's still colorless. 


   
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johan
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Posted by: @j

@jcancom 

Aparently because of the AA/DHA, note that as mentioned in the other thread I got greenish urine this morning and in fact also in the afternoon. But I've attached a pic I just took from the 25mg bottle that can be seen here (at the bottom of the page). After vigorous shaking, it's still colorless. 

The 500ML AA+MB bottle is actually a very convenient way to use the MB, the solution looks like water just a slight light blue reflection and it tastes both salty and sour. I use a small measuring cup (30ML) 20ML in this solution equals approx 1MG MB (LMB +DHA). I didn't see any coloring of urine at the 1MG dose. I just drank 10ML (0,5MG) and already feel a slight tingling in my head, lol! 


   
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Jcancom
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@johan, I want to be clear about your dosing of leucoMB. You have been taking ~ 1mg of MB with AA --> ~ 1 mg leucoMB? Have you went up as high as 15 mg leucoMB as suggested in some of your posts? 


   
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johan
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@jcancom I have been taking 1mg and one day 5mg, I'm going to test various doses, including the 15 mg which was used in the study for depression. But I've noticed this substance is quite powerful and earlier today I made a dropper in every 8 drops is about 100 mcg (micrograms), and even at that dose, I got some tinkling in the chin. I also made a spray and applied it on my hands and then a got tinkling in my upper arm.

The higher does seem to be for more acute illnesses such as sepsis, or poisoning, or covid. For cancer, you could apply low doses as antioxidants and high doses as prooxidants. Although I don't know what a high dose would be,  most of what I've read seems to point to 1mg/kilo or above as a high dose but I've other information points to 1 or 2 mg total as high enough. 

In any case, MB is very interesting because it can raise oxygen and ATP pretty fast and also has an antiestrogenic effect.


   
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Jcancom
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@j Thank you so much johan! Your description of reducing methylene blue with ascorbic acid has convinced me to give it a try. I have from time to time experimented with methylene blue, though the leuco form is obviously a much more powerful chemical formulation-- from what I understand leuco-MB is ~10 fold more powerful. Funnily, enough the selling material for MB speaks of it being a treatment for the virus and respiratory diseases!

Perhaps if we do some experimenting we will be able to think up some ways to make it even better? possibly we could even put the leucoMB into capsules? Might be interesting if we could take a blood draw and then do a spectroscopic analysis to see whether the leucoMB were still leucoMB once it reached the blood supply.

Best Wishes, Jcancom

 


   
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johan
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Posted by: @jcancom

 

Perhaps if we do some experimenting we will be able to think up some ways to make it even better? possibly we could even put the leucoMB into capsules? 

the AA/MB solution seems very stable, and definitely worth exploring further. 


   
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johan
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Posted by: @j
Posted by: @jcancom

 

Perhaps if we do some experimenting we will be able to think up some ways to make it even better? possibly we could even put the leucoMB into capsules? 

the AA/MB solution seems very stable, and definitely worth exploring further. 

Oh, and MB can help reduce lactic acid! Once the KWATCH you talked about some time ago is finally on sale we could do interesting experiments

https://www.pkvitality.com/ktrack-athlete/

Combining lipoic acid to methylene blue reduces the Warburg effect in CHO cells: From TCA cycle activation to enhancing monoclonal antibody production

https://pubmed.ncbi.nlm.nih.gov/32298377/


   
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Jcancom
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"Oh, and MB can help reduce lactic acid! Once the KWATCH you talked about some time ago is finally on sale we could do interesting experiments."

Whoa! That could be extremely interesting! MB could reduce lactic acid? Lactic acid is such a central feature of cancer-- it is the most conspicuously present change in biochemistry. Imagine if there were some catalyst that could do this lactic acid reduction! What might the cancer response be if you could simply clear out the body's excess lactic acid with MB/catalyst?

Yes, thank you for reminding me about the Kwatch. I am excited about the potential application of that technology. Just think if cancer patients had basically a 24/7 real time biomarker readout of the state of their cancer. Even now we have not really been getting it right with our anti-cancer strategy because we do not have a good feel for essential cancer features such as glucose, lactate, ketones. Having a readout on these could be invaluable.

Great news! I received the MB and AA!!!! Very excited about this. One thing I am unsure about is how to prepare a smaller batch. The MB is fractionated into 1 gram vials. Using 10 mg doses would last me 3 months if I put the full 1 gram into a plastic bottle. I would prefer if I could only use 100 mg of MB to make ~ 1 week supply. I am not sure though how I would measure out 100 mg.

 

Thank you again johan! This MB purchase means a lot to me. 

 

 

 


   
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johan
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I am not sure though how I would measure out 100 mg.

@jcancom  does this help? (see attachment)


   
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johan
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I'm using a 30ml dropper (16 x 1% MB drops in 30ml of water dissolved with approx 1 gram of ascorbic acid to make the solution turn colorless. That's 8mg MB in a 30ml bottle, and each full dropper approx 0,5mg (500mcg) of Leucomethylene Blue, or about 12,5mcg per drop, or 100mcg every 8 drops.)


   
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Jcancom
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@johan, I am very very pleased. I have the MB and I have the AA.

The problem that I had with the MB was it was divided into 1 gram vials. I just did not want to take the entire 1 gram and add water. This would be too much and I would worry that it might "go off" after so much time. I wanted to keep it as a powder as much as possible.

It really is not that easy to see what one might do to correctly measure out 10 mg draws from the 1 gram. However, I think that I might have the perfect workaround: I have noticed online that there are electronic scales that are accurate down to +/- 5 mg. If I were to measure out 100 mg (which would be  100 + /- 5 mg = (95, 105 ) mg, I could then take this quantity and add water (say 100 ml). Now 10 ml will contain 10 +/- 0.5 mg MB (i.e., (9.5,10.5) mg). That would be great!    

I think to hand it properly I should buy some of those mg dispensing spoons and possibly some dropper bottles. 

This is great! When I add in the AA to 10 mg MB I will then have ~ 10 mg leuoMB!

 

 

 


   
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Jcancom
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Yeah! I just downed 10 mg of leucoMB with the AA. I seemed to have stir in quite a bit of the AA for the blue to mostly fully disappear. It disappeared mostly after about 1 gram, but I think that I added in more like 2-3 grams to make the lingering blue tinge more fully disappear. 

I had already had some of the Cordon Blue MB (i.e., fish tank MB), though it tasted quite bitter without the AA. Yet, with the leucoMB with added AA it actually tasted quite nice; not bitter, unpleasant at all. I really should not have drank the full 10 mg straight out; that is not smart-- always best to dose up gradually. I will see what effect this might have if any. 10 mg leucoMB is = ~ 100 mg MB is a fair dose.

Will be very interested to see if this has any effect on my respiratory problems!

When you actually get around to trying this, it was not as messy or technically complex as I worried it might be.

Thank you johan!


   
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johan
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Posted by: @jcancom

Yeah! I just downed 10 mg of leucoMB with the AA. I seemed to have stir in quite a bit of the AA for the blue to mostly fully disappear. It disappeared mostly after about 1 gram, but I think that I added in more like 2-3 grams to make the lingering blue tinge more fully disappear. 

I had already had some of the Cordon Blue MB (i.e., fish tank MB), though it tasted quite bitter without the AA. Yet, with the leucoMB with added AA it actually tasted quite nice; not bitter, unpleasant at all. I really should not have drank the full 10 mg straight out; that is not smart-- always best to dose up gradually. I will see what effect this might have if any. 10 mg leucoMB is = ~ 100 mg MB is a fair dose.

Will be very interested to see if this has any effect on my respiratory problems!

When you actually get around to trying this, it was not as messy or technically complex as I worried it might be.

Thank you johan!

If the oral route doesn't help with respiratory symptoms you could try 0.1mg as nebulization, which is a part of a protocol used for the virus, combined with oral for support.

You did start with quite a high dose. I think 500mcg is good enough for a first dose and then up-titrate to 1 to 2mg. 

I agree, once you see the dark blue color turning almost colorless and taste it, it becomes quite a pleasant experience. It does stain though, even at the very light blue shade.


   
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johan
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Posted by: @jcancom

I think to hand it properly I should buy some of those mg dispensing spoons and possibly some dropper bottles. 

This is great! When I add in the AA to 10 mg MB I will then have ~ 10 mg leuoMB!

 

the tiny spoons (approx 30mg) and dropper bottles are really useful! well done J! 


   
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Jcancom
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@johan, after dosing with 10 mg LeucoMB I seemed to feel almost immediately better. I have been struggling for years not only with the respiratory problems but what seemed a host of related problems. I had a lot of stiffness, and what seemed to be nearly body wide inflammation, brain fog, trouble standing, every time I peed it had a stinging feeling, etc.. I wasn't sure what it was. The respiratory problems only seemed to be one part of a larger problem.

It is one of those problems where you go to the doctor and they are just not sure what to make of it.   I thought it might be something like long COVID. Strangely, much of these symptoms have now resolved. It is not always easy to be sure, though it does feel as though they are better. Fortunately, I have the spirometer, so I can give a numerical readout on my breathing. Admittedly, my breathing has not instantly improved, though the improvement in the other symptoms suggest that perhaps over time even the breathing will become less effortful.

Thank you johan!!!

 


   
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johan
(@j)
Joined: 4 years ago
Posts: 1199
 
Posted by: @jcancom

@johan, after dosing with 10 mg LeucoMB I seemed to feel almost immediately better. I have been struggling for years not only with the respiratory problems but what seemed a host of related problems. I had a lot of stiffness, and what seemed to be nearly body wide inflammation, brain fog, trouble standing, every time I peed it had a stinging feeling, etc.. I wasn't sure what it was. The respiratory problems only seemed to be one part of a larger problem.

It is one of those problems where you go to the doctor and they are just not sure what to make of it.   I thought it might be something like long COVID. Strangely, much of these symptoms have now resolved. It is not always easy to be sure, though it does feel as though they are better. Fortunately, I have the spirometer, so I can give a numerical readout on my breathing. Admittedly, my breathing has not instantly improved, though the improvement in the other symptoms suggest that perhaps over time even the breathing will become less effortful.

Thank you johan!!!

 

That's wonderful J!! One thing I immediately noticed is how fast this leucoMB//DHA works, so your experience matches mine. My sleep has improved since I have experimented with it, which is very nice for the insomniac I am. I haven't tried over 5mg because I drink 7 cups of coffee a day and I noticed I got a bit agitated when I took 5mg during the day, probably raises my blood pressure a bit too much in combination (not sure I will measure this as soon as I get a bp monitor).

There are so many interesting studies on MB, I also like the fact that it can reverse age-related problems (we're not getting any younger).

Methylene blue inhibits Caspase-6 activity, and reverses Caspase-6-induced cognitive impairment and neuroinflammation in aged mice

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915996/

Hope you continue to notice improvements J!

 


   
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johan
(@j)
Joined: 4 years ago
Posts: 1199
 

@jcancom

here's another option that might help with your breathing issue:

https://www.youtube.com/watch?v=TAHhe7hJ0Ug

 

 


   
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