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TNBC / testing strategies in a NED situation

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(@chris48)
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@j She was only taking the meds from the COC protocol (Mebendazol, Doxy, Atorvastatin, Metformin) + Luteolin, Apigenin, Bioperine and Berberine.


   
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(@j)
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@chris48 

I've posted this before:

https://www.sciencedirect.com/science/article/pii/S0753332221009525

"Co-treatment with mebendazole completely eliminates anticancer effect of the disulfiram and metformin combination. Tumor progression inhibited by disulfiram and metformin combination was completely rescued by mebendazole"

Metformin (an AMPK activator) and AMPK signaling:

https://bmccancer.biomedcentral.com/articles/10.1186/s12885-022-09211-1

Long-term Doxycycline use reduces butyrate:

https://www.jidonline.org/article/S0022-202X(18)30615-8/fulltext
https://synergiesforcancertreatments.blogspot.com/p/butyrate.html

Since you have apigenin in the protocol I'd add curcumin.

https://pubmed.ncbi.nlm.nih.gov/23485682/
https://ar.iiarjournals.org/content/41/3/1271

 


   
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(@chris48)
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Many thanks Johan!

Posted by: @j

"Co-treatment with mebendazole completely eliminates anticancer effect of the disulfiram and metformin combination. Tumor progression inhibited by disulfiram and metformin combination was completely rescued by mebendazole"

Yes, I remember. I showed it also to Bianca and her oncologist from the COC and asked him if it is possible to leave it out or replace it with Berberine. He said that Metformin has a crucial role in the protocol and she should definetely continue to take it. Therefore she sticked to it.

Anyway, if the CTC keep increasing it might be better to try something else...


   
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(@j)
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@chris48 

Honokiol inhibits the growth of hormone-resistant breast cancer cells: its promising effect in combination with metformin
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10568957/

Synergy between sublethal doses of shikonin and metformin fully inhibits breast cancer cell migration and reverses epithelial-mesenchymal transition
https://www.researchgate.net/publication/360453260_Synergy_between_sublethal_doses_of_shikonin_and_metformin_fully_inhibits_breast_cancer_cell_migration_and_reverses_epithelial-mesenchymal_transition

Synergistic Anti-proliferative Effects of Metformin and Silibinin Combination on T47D Breast Cancer Cells via hTERT and Cyclin D1 Inhibition
https://pubmed.ncbi.nlm.nih.gov/29920623/

Metformin combined with quercetin synergistically repressed prostate cancer cells via inhibition of VEGF/PI3K/Akt signaling pathway
https://pubmed.ncbi.nlm.nih.gov/29678660/

Synergistic Anti-Cancer Effect of Baicalein and Metformin against Human Lung Cancer
https://www.researchgate.net/publication/320527468_Synergistic_Anti-Cancer_Effect_of_Baicalein_and_Metformin_against_Human_Lung_Cancer


   
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(@chris48)
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@j Many thanks Johan! I have just ordered Honokiol (liposomal and regular version) from MCS. We will add it to the protocol.


   
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(@j)
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@chris48 sounds good, Chris.


   
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(@chris48)
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I am also thinking about adding magnolol as it showed synergistic effects with honokiol: https://www.oncotarget.com/article/8674/text/


   
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Posted by: @chris48

I am also thinking about adding magnolol as it showed synergistic effects with honokiol: https://www.oncotarget.com/article/8674/text/

Yes, Chris, that's a good one. Check my blog for more combinations that might be a good fit for your protocol:

https://synergiesforcancertreatments.blogspot.com/2020/11/combination-of-natural-supplements-as.html

 


   
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(@j)
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@chris48 She was taking aspirin before, I'd consider adding it to her protocol again:

Aspirin / Metformin
https://www.nature.com/articles/srep13390
https://www.sciencedirect.com/science/article/pii/S1936523321002011


   
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(@chris48)
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@j Yes, we have that in the protocol, but we were just discussing if she should take it. This info makes a pretty good case for it. Thank you!🙏


   
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Posted by: @chris48

@j Yes, we have that in the protocol, but we were just discussing if she should take it. This info makes a pretty good case for it. Thank you!🙏

Combination of Thymoquinone and Intermittent Fasting as a Treatment for Breast Cancer Implanted in Mice

https://www.mdpi.com/2223-7747/13/1/35

Therapeutic Potential of Thymoquinone in Triple-Negative Breast Cancer Prevention and Progression through the Modulation of the Tumor Microenvironment

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746460/

Have a great weekend!

 


   
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(@j)
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@chris48 

Artemisinin induces selective and potent anticancer effects in drug resistant breast cancer cells by inducing cellular apoptosis and autophagy and G2/M cell cycle arrest
https://pubmed.ncbi.nlm.nih.gov/32862573/


   
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(@j)
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Since you're considering adding Magnolol to your protocol I think you'll find the following studies interesting:

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520007/

Azithromycin, an antibiotic, inhibits autophagy. ( https://www.tocris.com/cell-biology/autophagy/inhibitors)

Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy

https://www.researchgate.net/publication/352468496_Synergistic_effects_of_autophagymitophagy_inhibitors_and_magnolol_promote_apoptosis_and_antitumor_efficacy

And, in this study, they found autophagy accelerated the aggressiveness of TNBC. They concluded that inhibition of autophagy or Fas signaling may provide novel targets for TNBC therapy.

https://pubs.rsc.org/en/content/articlelanding/2014/fo/c4fo00145a

 

 

 

 

 


   
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(@j)
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@chris48

Posted by: @j

Since you're considering adding Magnolol to your protocol I think you'll find the following studies interesting:

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520007/

Azithromycin, an antibiotic, inhibits autophagy. ( https://www.tocris.com/cell-biology/autophagy/inhibitors)

Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy

https://www.researchgate.net/publication/352468496_Synergistic_effects_of_autophagymitophagy_inhibitors_and_magnolol_promote_apoptosis_and_antitumor_efficacy

And, in this study, they found autophagy accelerated the aggressiveness of TNBC. They concluded that inhibition of autophagy or Fas signaling may provide novel targets for TNBC therapy.

https://pubs.rsc.org/en/content/articlelanding/2014/fo/c4fo00145a

 

 

 

 

 

 


   
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(@j)
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I messed up the link to the autophagy inhibitors page. Here it is:

https://www.tocris.com/cell-biology/autophagy/inhibitors

 


   
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(@chris48)
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Many thanks again, @Johan! Looks very interesting! Unfortunately we would need to find someone who is willing to prescribe azithromycin. Also I am a bit worried about the side effects and effect on the microbiom if she took 2 antibiotics concurrently. But we will definetely keep it on the list.


   
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(@j)
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Oleate Promotes Triple-Negative Breast Cancer Cell Migration by Enhancing Filopodia Formation through a PLD/Cdc42-Dependent Pathway

https://www.mdpi.com/1422-0067/25/7/3956


   
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(@j)
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Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways

https://journals.lww.com/md-journal/fulltext/2024/02230/curcumin_suppresses_metastasis_of_triple_negative.5.aspx


   
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(@chris48)
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Posted by: @j

 

Oleate Promotes Triple-Negative Breast Cancer Cell Migration by Enhancing Filopodia Formation through a PLD/Cdc42-Dependent Pathway

https://www.mdpi.com/1422-0067/25/7/3956

Thank you, Johan! I am wondering whether that should impact her diet since oleate is found in very healthy foods like olive oil, avocados and nuts. Usually those are rather known as anti-cancer foods. Would you rather limit the intake of those foods or would you consider this as an overreaction to a cell study which not necessarily translates into the same activity of those specfific foods holistically in the body? I have e.g. also found a study that rather points to olive oil constituents having an inhibiting effect on TNBC cells: https://link.springer.com/article/10.1007/s10142-023-01230-w  

 


   
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(@j)
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@chris48 

I don't know, Chris. It does seem easy to get a little too much of it. 

Here’s a list of the top 20 foods and oils high in oleic acid and the percentage of the acid that makes up the
total fat content:

Olive oil: 80 percent
Almond oil: 80 percent
Hazelnuts: 79 percent
Apricot kernel oil: 70 percent
Avocado oil: 65 percent to 70 percent
Pecans: 65 percent
Almonds: 62 percent
Macadamia nuts: 60 percent
Cashews: 60 percent
Cheese: 58 percent
Beef: 51 percent
Sweet almond oil: 50 percent to 85 percent
Emu oil: 48 percent
Eggs: 45 percent to 48 percent
Argan oil: 45 percent
Sesame oil: 39 percent
Milk: 20 percent
Sunflower oil: 20 percent
Chicken: 17 percent
Grapeseed oil: 16 percent

Source: https://draxe.com/nutrition/oleic-acid/

 

 

 


   
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(@j)
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Coconut oil has almost no oleic acid. 


   
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@chris48

High Metastaticgastric and Breast Cancer Cells Consume Oleic Acid in an AMPK Dependent Manner

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097330

"We reported that AMP-activated protein kinase (AMPK) was obviously activated in highly aggressive carcinoma cell lines treated by oleic acid, including gastric carcinoma HGC-27 and breast carcinoma MDA-MB-231 cell lines. AMPK enhanced the rates of fatty acid oxidation and ATP production and thus significantly promoted cancer growth and migration under serum deprivation."

 


   
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(@j)
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Oleic acid from cancer-associated fibroblast promotes cancer cell stemness by stearoyl-CoA desaturase under glucose-deficient condition

https://cancerci.biomedcentral.com/articles/10.1186/s12935-022-02824-3


   
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(@j)
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Here's another study pointing to cancer proliferation due to Oleic Acid.

"OA stimulates cell proliferation and glycolysis pathways via PPARα activation"

https://www.sciencedirect.com/science/article/abs/pii/S0006291X23003431

Fats and cancer, no bueno.

 


   
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(@j)
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Cancer depends on fatty acids for ATP production: A possible link between cancer and obesity
https://www.sciencedirect.com/science/article/pii/S1044579X22001766

Highlights

All living cells produce ATP in mitochondria using the TCA cycle or fatty acid oxidation (FAO).

However, cancer cells do not use carbohydrates for the TCA cycle because of the Warburg effect.

Therefore, cancer cells must rely on OxPhos with the ETC using fatty acids supplied from blood vessels to produce ATP.


Docosahexaenoic acid appears to be the outlier.

I've mentioned it many times before, but based on the scientific and anecdotal evidence a ketogenic diet doesn't appear to be an effective anticancer diet and may actually promote cancer.


   
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(@j)
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Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia

https://pubmed.ncbi.nlm.nih.gov/37311455/

"delayed tumor growth but accelerated cachexia onset and shortened survival in mice fed KD."


   
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(@j)
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Aspirin vs Placebo as Adjuvant Therapy for Breast Cancer The Alliance A011502 Randomized Trial

Key Points

Question

Does aspirin at 300 mg/d improve invasive disease-free survival among survivors of nonmetastatic breast cancer?

Findings

This randomized, placebo-controlled clinical trial included 3020 patients with high-risk nonmetastatic breast cancer. The trial was terminated early because of lack of benefit from aspirin (hazard ratio for invasive disease-free survival for aspirin vs placebo, 1.27 [not statistically significant]).

There was no benefit of aspirin at 300 mg/d on breast cancer recurrence and survival.

https://jamanetwork.com/journals/jama/fullarticle/2818110


   
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jens
 jens
(@jens)
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@chris48 

Hi Chris.

It's been a awhile since I posted. I am always thinking of everyone here. It was six months since Lourdes passed away. I think I am ready to start helping in any way possible.

I saw the other day you were hesitant adding Azithromycin with the Doxycycline and IVC.  The Doxycycline targets large mitochrondrial ribosome and Azithromycin targets the small mitrochrondial ribosome. I think its important to target both. I know a doctor thats using both with success. 

 

Jens

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520007/

 

 

 

 

 

 

 

 

 

 


   
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 jens
(@jens)
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@chris48 

Hi Chris.

It's been a awhile since I posted. I am always thinking of everyone here. It was six months since Lourdes passed away. I think I am ready to start helping in any way possible.

I saw the other day you were hesitant adding Azithromycin with the Doxycycline and IVC.  The Doxycycline targets large mitochrondrial ribosome and Azithromycin targets the small mitrochrondial ribosome. I think its important to target both. I know a doctor thats using both with success. 

 

Jens

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520007/

 

 

 

 

 

 

 

 

 

 


   
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(@j)
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@jens good to hear from you Jens, hope you and the children are doing okay.


   
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